The activity in ex vivo expansion of cord blood myeloid progenitor cells before and after cryopreservation

Acta Haematologica
S Y WangC K Ho

Abstract

A total of 50 human umbilical cord blood (UCB) samples were studied. The hematopoietic stem/progenitor (CD34+) populations were isolated from UCB mononuclear cells (MNC) by means of immunomagnetic separation. Double immunofluorescent staining of UCB CD34+ cells revealed that there was a high proportion (82.33 +/- 4.47%) of CD34+ cells co-expressing CD13, while the percentage of CD34+ CD33+ cells was much lower (22.17 +/- 3.35%). In contrast, for co-expressing lymphoid differentiation antigens, the proportion of CD34+CD38+ cells (38.34 +/- 6.09%) was relatively higher than that of CD34+CD10+ cells (11.52 +/- 1.24%) or CD34+CD2+ cells (9.84 +/- 2.30%). For stimulating the ex vivo expansion of UCB progenitor cells, no single hematopoietic growth factor (HGF) was efficacious when used alone, while combination of 4 HGFs, such as GM-CSF, G-CSF, IL-3, and SCF could induce a 55-fold increase in the myeloid progenitor cells, day-14 CFU-GM, in a short term of 7 days' liquid culture. Cryopreservation of UCB as MNC preparations at -196 degrees C could satisfactorily retain the number and activity of CD34+ cells. After thawing, a high recovery rate of about 80% CD34+ cells was obtained. When suspended in liquid cultures containing a combina...Continue Reading

Citations

Sep 6, 2003·Journal of Immunological Methods·A CostantiniM Montroni
Dec 18, 2003·Clinical and Experimental Pharmacology & Physiology·J J Zwaginga, P Doevendans
Jun 6, 2006·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Azza A MohamedRaafat M Riad
Jul 8, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M L L F ChauffailleJ Kerbauy
Feb 3, 2007·American Journal of Hematology·David BerzPeter J Quesenberry

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