The Activity-Induced Long Non-Coding RNA Meg3 Modulates AMPA Receptor Surface Expression in Primary Cortical Neurons

Frontiers in Cellular Neuroscience
Men C TanVictor Anggono

Abstract

Transcription of new RNA is crucial for maintaining synaptic plasticity, learning and memory. Although the importance of synaptic plasticity-related messenger RNAs (mRNAs) is well established, the role of a large group of long non-coding RNAs (lncRNAs) in long-term potentiation (LTP) is not known. In this study, we demonstrated the expression of a lncRNA cluster, namely maternally expressed gene 3 (Meg3), retrotransposon-like gene 1-anti-sense (Rtl1-AS), Meg8 and Meg9, which is located in the maternally imprinted Dlk1-Dio3 region on mouse chromosome 12qF1, in primary cortical neurons following glycine stimulation in an N-Methyl-D-aspartate receptor (NMDAR)-dependent manner. Importantly, we also validated the expression of Meg3, Meg8 and Meg9 in the hippocampus of mice following cued fear conditioning in vivo. Interestingly, Meg3 is the only lncRNA that is expressed in the nucleus and cytoplasm. Further analysis revealed that Meg3 loss of function blocked the glycine-induced increase of the GluA1 subunit of AMPA receptors on the plasma membrane, a major hallmark of LTP. This aberrant trafficking of AMPA receptors correlated with the dysregulation of the phosphatidylinoside-3-kinase (PI3K)/AKT signaling pathway and the downregula...Continue Reading

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Citations

Feb 15, 2020·Journal of Biochemical and Molecular Toxicology·Li Ji, Li Ma
Feb 14, 2020·Cellular and Molecular Neurobiology·Jocelyn WidagdoVictor Anggono
Sep 6, 2019·Scientific Reports·Hamid FallahSoudeh Ghafouri-Fard
Apr 20, 2018·Non-coding RNA·Diana Pereira FernandesGuy Barry
Apr 17, 2018·Non-coding RNA·Amanda Salviano-SilvaMaria Luiza Petzl-Erler
Jun 1, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Aree WanasuntronwongMayuree H Tantisira
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Oct 29, 2020·Cell Reports·Xuan Ling Hilary YongVictor Anggono
Feb 3, 2018·Physiology & Behavior·Julianna N BrutmanJon F Davis
May 25, 2021·Frontiers in Molecular Neuroscience·Sergio Espeso-GilStephan Ossowski
Jun 12, 2021·Metabolic Brain Disease·Mohammad TaheriMir Davood Omrani
Jun 4, 2020·Aging and Disease·Cristina-Sorina CatanaIoana Berindan-Neagoe

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Methods Mentioned

BETA
gene trap
antisense oligonucleotide
PCR

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