PMID: 9525637Apr 3, 1998Paper

The activity of Sendai virus genomic and antigenomic promoters requires a second element past the leader template regions: a motif (GNNNNN)3 is essential for replication

Journal of Virology
C TapparelL Roux

Abstract

The paramyxovirus genome, a nonsegmented, negative-polarity, single-stranded RNA of approximately 15 kb, contains six transcription units flanked at the 3' and 5' ends by a short (approximately 50- to 60-nucleotide) extracistronic sequence, dubbed the positive and negative leader regions. These leader template regions, present at the 3' end of the genome and the antigenome, have been shown to contain essential signals governing RNA replication activity. Whether they are sufficient to promote replication is still open to question. By using a series of Sendai virus defective interfering RNAs carrying a nested set of deletions in the promoter regions, it is shown here that for both the genomic and antigenomic promoters, a 3'-end RNA sequence of 96 nucleotides is required to allow replication. Sequence comparison of active and inactive promoters led to the identification of a set of three nucleotide hexamers (nucleotides 79 to 84, 85 to 90, and 91 to 96) containing a repeated motif RXXYXX [shown as 5'-3' positive-strand]. Sequential mutation of each hexamer into its complementary sequence confirmed their essential role. The three hexamers are required, and their relative positioning is important, since displacing them by 6 nucleoti...Continue Reading

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Citations

Oct 22, 2002·The Journal of General Virology·Gabriele NeumannYoshihiro Kawaoka
Dec 18, 2004·The Journal of General Virology·Diane VulliémozLaurent Roux
Jul 30, 2005·Journal of Virology·Michael WeikElke Mühlberger
Jun 14, 2015·FEBS Letters·Sonia Longhi
Aug 9, 2020·Molecular Genetics and Genomics : MGG·Lian Yih PongNazlina Ibrahim
Jun 2, 2015·Frontiers in Microbiology·Robert Cox, Richard K Plemper
Apr 20, 2001·Journal of Virology·D Vulliémoz, L Roux
Jul 19, 2002·The Journal of General Virology·David BhellaRobert P Yeo
Mar 24, 2005·The Journal of General Virology·Ashley C BanyardThomas Barrett
May 7, 2002·Journal of Virology·Philippe Le MercierDaniel Kolakofsky
Jun 17, 2005·The Journal of General Virology·Daniel KolakofskyRob W H Ruigrok
Dec 4, 2012·Viruses·Kristina BrauburgerJudith Olejnik
Feb 25, 2005·FEMS Microbiology Letters·Pramila Walpita, Ramon Flick
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sarah L NotonRachel Fearns
Apr 14, 2016·The Journal of General Virology·Yusuke MatsumotoMachiko Nishio
Nov 21, 2001·The Journal of General Virology·V MiouletM D Baron
Jun 9, 2006·The Journal of General Virology·Vanessa M CowtonRachel Fearns
Feb 16, 2006·The Journal of General Virology·Samuel Cordey, Laurent Roux
Jan 24, 2018·RNA·Yusuke MatsumotoMachiko Nishio
Aug 19, 2007·The Journal of General Virology·Pramila Walpita, Clarence J Peters
Nov 6, 2020·PLoS Pathogens·Irina GutscheDaniel Kolakofsky
Dec 28, 2018·RNA·Philippe le Mercier, Daniel Kolakofsky
Feb 28, 2016·Current Opinion in Virology·Sham NambulliW Paul Duprex

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