The actomyosin ring recruits early secretory compartments to the division site in fission yeast

Molecular Biology of the Cell
Aleksandar VjesticaSnezhana Oliferenko

Abstract

The ultimate goal of cytokinesis is to establish a membrane barrier between daughter cells. The fission yeast Schizosaccharomyces pombe utilizes an actomyosin-based division ring that is thought to provide physical force for the plasma membrane invagination. Ring constriction occurs concomitantly with the assembly of a division septum that is eventually cleaved. Membrane trafficking events such as targeting of secretory vesicles to the division site require a functional actomyosin ring suggesting that it serves as a spatial landmark. However, the extent of polarization of the secretion apparatus to the division site is presently unknown. We performed a survey of dynamics of several fluorophore-tagged proteins that served as markers for various compartments of the secretory pathway. These included markers for the endoplasmic reticulum, the COPII sites, and the early and late Golgi. The secretion machinery exhibited a marked polarization to the division site. Specifically, we observed an enrichment of the transitional endoplasmic reticulum (tER) accompanied by Golgi cisternae biogenesis. These processes required actomyosin ring assembly and the function of the EFC-domain protein Cdc15p. Cdc15p overexpression was sufficient to ind...Continue Reading

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Citations

Aug 21, 2013·PLoS Genetics·Ling-Ling SunLi-Lin Du
Jul 10, 2009·PloS One·Jae-Sook ParkSean M Hemmingsen
Dec 10, 2009·The Journal of Cell Biology·Gordon PolevoyJulie A Brill
Feb 18, 2015·The Journal of Cell Biology·Alaina H WilletKathleen L Gould
Nov 11, 2015·The Journal of Cell Biology·Pranav UllalSophie G Martin
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May 4, 2010·Current Biology : CB·Dan ZhangSnezhana Oliferenko
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Oct 11, 2019·The Journal of Cell Biology·Mariona RamosJuan Carlos Ribas
Dec 3, 2011·Traffic·Yasuyuki Suda, Akihiko Nakano
Feb 20, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Thi Thuy Trang NguyenEe Sin Chen
Apr 29, 2015·The Journal of Biological Chemistry·Sumana Raychaudhuri, Peter J Espenshade
May 16, 2015·Journal of Cell Science·Rajesh Arasada, Thomas D Pollard

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