The acute effect of FK506 and cyclosporine on endothelial cell function and renal vascular resistance

Transplantation
A BenigniG Remuzzi

Abstract

We have previously documented that cyclosporine exerts a direct cytotoxic effect on endothelial cells and causes an increase in renal vascular resistance (RVR) in the rat. In the present study we investigated whether FK506, a novel immunosuppressive agent thought to be less nephrotoxic than CsA, impairs endothelial cell function in vitro and affects RVR in vivo. In vitro eicosanoid release and endothelin release were measured in bovine aortic endothelial cells in culture exposed for 1, 6, and 24 hr to increasing concentrations of FK506 (1 nM to 10 microM) or CsA (0.5, 10 microM). No significant changes in TxB2 and 6-keto-PGF1 alpha (the stable breakdown products of TxA2 and PGI2, respectively) and endothelin release were found after 1 and 6 hr of incubation with all the concentrations of FK506 and CsA considered. FK506 did not affect endothelin release even after 24 hr of incubation. In contrast, cell exposure to CsA was associated with a dose-dependent increase in TxB2, 6-keto-PGF1 alpha, and endothelin release that reached statistical significance after incubation with 10 microM CsA. FK506 did not induce cell detachment or lysis at any concentration and time considered, while 10 microM CsA induced a significant reduction in c...Continue Reading

References

Apr 27, 2010·Der Nervenarzt·T LimanM Endres
Mar 28, 2008·Current Oncology Reports·Christopher VaughnDavid Schiff
Apr 1, 1994·Trends in Pharmacological Sciences·I Chapman, L Mazzoni
Aug 15, 2001·European Journal of Pharmacology·K K HwangH V Sparks
Sep 28, 1999·European Journal of Pharmacology·T A MarsenC A Baldamus
May 30, 2001·Transplantation Proceedings·T NakataniT Kishimoto
Sep 2, 1998·Transplantation Proceedings·P A Keown, D R Primmett
Sep 17, 2002·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Hugues JeanmartLouis P Perrault
Apr 12, 2003·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Louis P PerraultPaul M Vanhoutte
Apr 14, 1999·Kidney International·J J De LimaJ B Roullet
Feb 22, 1996·The New England Journal of Medicine·J HincheyL R Caplan
May 22, 1998·Journal of Cardiovascular Pharmacology·K YokokawaJ Yoshikawa
May 29, 2004·Transplantation·Brian J NankivellSimon M Gruenewald
Sep 1, 1995·Clinical and Experimental Pharmacology & Physiology·T F AndohW M Bennett
May 1, 1996·International Journal of Urology : Official Journal of the Japanese Urological Association·S MugiyaA Takada
Oct 13, 2000·Fundamental & Clinical Pharmacology·T A MarsenC A Baldamus
Oct 27, 2001·Journal of Neuroimaging : Official Journal of the American Society of Neuroimaging·P W SchaeferL H Schwamm
Sep 1, 1995·Postgraduate Medical Journal·R M Jindal, I Popescu
Mar 28, 1998·Japanese Journal of Pharmacology·J UchidaT Kishimoto
Sep 22, 2007·AJR. American Journal of Roentgenology·A M McKinneyMehmet Teksam
Apr 13, 1996·Lancet·G A Donnan
Dec 29, 1998·Transplantation Proceedings·L C Paul
Nov 13, 2009·Kidney International·Kirsten MadsenBoye L Jensen
Mar 31, 1998·The American Journal of the Medical Sciences·M S CainR F Holcombe
Mar 20, 2012·Clinical Breast Cancer·Yen-Hao Chen, Cheng-Hua Huang
Jan 28, 2004·British Journal of Pharmacology·S M GardinerT Bennett
May 30, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Anja Susanne MühlfeldJürgen Floege
Nov 14, 2015·AJNR. American Journal of Neuroradiology·S J KariaA M McKinney
Feb 18, 2009·Internal Medicine Journal·M A Marinella, R J Markert
Jul 1, 1994·Thrombosis Research·J MalyszkoA Takada
Sep 27, 2005·Transplantation Proceedings·T DervauxB Moulin
Jun 21, 2005·European Journal of Pharmacology·Ender TekesMeral Tuncer
Nov 30, 2006·Journal of Pharmacological Sciences·Guray SoydanMeral Tuncer
Mar 11, 2008·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jianping Li, Donna H Wang
Jun 4, 2013·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Eray YagmurAnja Susanne Mühlfeld

Related Concepts

Pathologic Cytolysis
Thromboxane A2
Exertion
Restasis
Vascular Resistance
Diastolic Blood Pressure
Cyclosporine
Bos taurus
Renal Vascular Disorder
Cell Count

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.