PMID: 2118294Jun 1, 1990Paper

The adaptation of histometry and immunohistochemical detection of fetal myosin as diagnostic methods for stress myopathy in swine

Zentralblatt für Veterinärmedizin. Reihe A
E M Breuer

Abstract

The aim of this study was to examine the reliability of histometric and immunocytochemical methods in the diagnosis of stress-induced myopathies in pigs. A total of 33 German Landrace and Mangalitza pigs ranging in age from 4 weeks to 1.5 years were used for the determination of creatine kinase (CK) activity in blood serum and the histological, histometrical and immunocytochemical examination of 10 different skeletal muscles. The results show that a computer-aided histometric analysis of skeletal muscle fibers with special regard to their coefficient of variation and the interquartile range is not valuable for the diagnosis of myopathy in pigs. This contradicts the results reported in other species, where the grade of myopathy was found to correlate positively with the coefficient of variation. Using an antibody against fetal skeletomuscular myosin in immunocytochemistry, an age-dependently varying mixture of small myofibers of a physiological tapered and a pathological regenerating type was observed in porcine muscles, resulting in a relatively constant coefficient of variation of between 30-40% in skeletal muscles over the entire age range examined. In healthy porcine skeletal muscles, fetal myosin is only expressed prenatall...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.