The adaptor protein AP-4 as a component of the clathrin coat machinery: a morphological study

The Biochemical Journal
Nicolas Barois, Oddmund Bakke

Abstract

The four members of the AP (adaptor protein) family are heterotetrameric cytosolic complexes that are involved in the intracellular trafficking of cargo proteins between different organelles. They interact with motifs present in the cytoplasmic tails of their specific cargo proteins at different intracellular locations. While AP-1, AP-2 and AP-3 have been investigated extensively, very few studies have focused on the fourth member, AP-4. In the present study, we report on the intracellular localization of AP-4 in the MDCK (Madin-Darby canine kidney) and MelJuSo cell lines after immunogold labelling of ultrathin cryosections. We find that AP-4 is localized mainly in the Golgi complex, as well as on endosomes and transport vesicles. Interestingly, we show for the first time that AP-4 is localized with the clathrin coat machinery in the Golgi complex and in the endocytic pathway. Furthermore, we find that AP-4 is localized with the CI-MPR (cation-independent mannose 6-phosphate receptor), but not with the transferrin receptor, LAMP-2 (lysosomal-associated membrane protein-2) or invariant chain. The difference in morphology between CI-MPR/AP-4-positive vesicles and CI-MPR/AP-1-positive vesicles raises the possibility that AP-4 acts...Continue Reading

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Citations

Apr 15, 2011·Molecular Biology of the Cell·Jason BurgessJulie A Brill
Feb 22, 2013·ELife·Bettina L LeeGregory M Barton
Aug 19, 2007·Seminars in Cell & Developmental Biology·Matthew J Paul, Lorenzo Frigerio
Nov 22, 2007·International Journal of Cancer. Journal International Du Cancer·Cathrine FladebyOddmund Bakke
Jan 10, 2013·Archives of Insect Biochemistry and Physiology·Yan-shan NiuYun-gen Miao
Apr 24, 2015·Frontiers in Cellular Neuroscience·Chan Choo Yap, Bettina Winckler
Apr 8, 2010·Immunobiology·Anat BenadoRonit Sagi-Eisenberg
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna P LawtonMitchell Kronenberg
Sep 9, 2006·Journal of Cell Science·Hiroshi Ohno
Nov 2, 2016·Journal of Cell Science·Cinzia Progida, Oddmund Bakke
Jun 2, 2005·Biochimica Et Biophysica Acta·Enrique Rodriguez-Boulan, Anne Müsch

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