The addition of docosahexaenoic acid (DHA) and antioxidants (glutathione peroxidase and superoxide dismutase) in extenders to epididymal sperm cryopreservation in bulls

Zygote : the Biology of Gametes and Early Embryos
João D A LosanoMarcilio Nichi

Abstract

SummaryThe cryopreservation of epididymal sperm is an important technique that allows genetic material to be preserved, even post mortem. However, cryopreservation leads to increased oxidative stress and impaired sperm viability. Polyunsaturated fatty acid (PUFA) supplementation may improve certain sperm characteristics, but it also makes sperm more susceptible to oxidative stress, therefore adding antioxidants that counteract oxidative stress has become an option. In this context, this study aimed to evaluate the effect of the interaction between docosahexaenoic acid (DHA) and antioxidants on the quality after the cryopreservation of epididymal bull sperm. Twenty epididymides were collected after slaughter, and epididymal sperm was cryopreserved with bovine extender supplemented with docosahexaenoic acid (DHA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). We verified an improvement in motility in the group that was treated only with DHA 5 µM and a concentration-dependent effect on susceptibility to lipid peroxidation that was associated with DHA concentration (1 µM, 5 µM or 10 µM). Moreover, treatment with DHA (5 µM) and SOD (20 IU/ml) resulted in higher sperm motility. Thus, the association between DHA (5 µM) ...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Jan 1, 1978·Methods in Enzymology·J A Buege, S D Aust
May 1, 1983·Andrologia·H P Nissen, H W Kreysel
Feb 29, 2000·Molecular Human Reproduction·A LenziM Picardo
Mar 7, 2000·Frontiers in Bioscience : a Journal and Virtual Library·A LenziE Panfili
Feb 28, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·J A RookeB K Speake
Mar 27, 2001·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·D Evenson, L Jost
May 17, 2003·Fertility and Sterility·Ashok AgarwalMohamed A Bedaiwy
Apr 28, 2004·Molecular and Cellular Endocrinology·P VernetJ R Drevet
Aug 10, 2006·The Journal of Clinical Endocrinology and Metabolism·R John AitkenEileen A McLaughlin
Jul 28, 2012·Reproduction in Domestic Animals = Zuchthygiene·F MinerviniV L Barile
Aug 11, 2012·Journal of Andrology·R John AitkenSarah A Robertson
Dec 27, 2012·The World Allergy Organization Journal·Esra BirbenOmer Kalayci
Aug 14, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Renata SimõesMayra Elena Ortiz Assumpção

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