Abstract
Tartrate-resistant acid phosphatase (TRAP) exists as two isoforms, 5a and 5b. TRAP 5a is elevated in adipose tissue of obese women, interacts with pre-adipocytes and is linked to insulin-sensitive hyperplastic obesity when overexpressed in mice. The aim of this study was to investigate the relation between serum TRAP 5a, adiposity indices and metabolic syndrome risk markers in lean and obese women, using a newly developed TRAP 5a-specific ELISA. A TRAP 5a sandwich ELISA was optimized using TRAP 5a-specific monoclonal antibodies and tested in sera of healthy males. TRAP 5a levels were quantitated in sera from healthy males and lean and obese women. Serum TRAP 5a protein levels were lower in obese women in comparison with lean. In obese, but not in lean women, serum TRAP 5a correlated positively to % fat mass, BMI, waist- and hip circumference, waist-to-hip ratio and PAI, while no correlations to serum leptin, HOMA, glucose, insulin, FFA, HDL, TG, APO-A1 and APO-B were observed. TRAP 5a serum levels correlated positively to anthropometric obesity parameters but not to metabolic syndrome risk factors, indicating that serum TRAP 5a is associated with pathological adipose tissue expansion.
References
Jan 15, 1997·The Biochemical Journal·B Ek-RylanderG Andersson
Feb 5, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C S BrehmeR Wolfert
Jul 30, 1999·Structure·L W GuddatJ L Martin
Jul 14, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J M HalleenH K Väänänen
Oct 6, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·K TakahashiL T Yam
May 2, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anthony J JanckilaLung T Yam
Jun 20, 2002·Calcified Tissue International·J M HalleenH K Väänänen
Aug 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anthony J JanckilaLung T Yam
Nov 16, 2004·Journal of Leukocyte Biology·Anthony J JanckilaLung T Yam
Jul 5, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Tsu-Yi ChaoAnthony J Janckila
Jul 12, 2005·Journal of Immunological Methods·Bartek ZuberNiklas Ahlborg
Dec 15, 2006·Nature·Gökhan S Hotamisligil
Feb 9, 2007·Diabetes·Luigi FontanaSamuel Klein
Mar 21, 2007·American Journal of Clinical Pathology·Anthony J JanckilaLung T Yam
Mar 6, 2008·PloS One·Pernilla LångPeter Arner
Apr 16, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Anthony J JanckilaLung T Yam
Sep 22, 2009·Metabolism: Clinical and Experimental·Kuang-Chung ShihTsu-Yi Chao
Nov 17, 2009·Calcified Tissue International·Anthony J Janckila, Lung T Yam
Feb 13, 2010·Biochimica Et Biophysica Acta·Serhan ZengerGöran Andersson
Oct 23, 2010·Clinical & Experimental Metastasis·Serhan ZengerGöran Andersson
Jan 27, 2011·Diabetologia·E KlimcakovaD Langin
Feb 9, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Anthony J JanckilaTsu-Yi Chao
Mar 10, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·P LångP Arner
May 19, 2011·Current Opinion in Clinical Nutrition and Metabolic Care·David L MorrisCarey N Lumeng
Jun 29, 2011·BMC Medical Genomics·Hong JiaoIngrid Dahlman
Dec 10, 2013·Biochimica Et Biophysica Acta·Christina PatlakaGöran Andersson
May 14, 2014·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Yi-Ying WuTsu-Yi Chao
Nov 20, 2014·European Journal of Human Genetics : EJHG·Hong JiaoIngrid Dahlman
Dec 3, 2014·Biochemical and Biophysical Research Communications·Christina PatlakaGöran Andersson
Nov 10, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kuang-Chung ShihTsu-Yi Chao
Mar 14, 2016·Eating Behaviors·Cecilia PetterssonHeléne Bertéus Forslund