The advantages of linear concentration-response curves for in vitro bioassays with environmental samples

Environmental Toxicology and Chemistry
Beate I EscherDaniel L Villeneuve

Abstract

In vitro assays and high-throughput screening (HTS) tools are increasingly being employed as replacements for animal testing, but most concentration-response curves are still evaluated with models developed for animal testing. We argue that application of in vitro assays, particularly reporter gene assays, to environmental samples can benefit from a different approach to concentration-response modeling. First, cytotoxicity often occurs at higher concentrations, especially for weakly acting compounds and in complex environmental mixtures with many components. In these cases, specific effects can be masked by cytotoxicity. Second, for many HTS assays, low effect levels can be precisely quantified because of the low variability of controls in cell-based assays and the opportunity to run many concentrations and replicates when using high-density well-plate formats (e.g., 384 or more wells per plate). Hence, we recommend focusing concentration-response modeling on the lower portion of the concentration-response curve, which is approximately linear. Effect concentrations derived from low-effect level linear concentration-response models facilitate simple derivation of relative effect potencies and the correct application of mixture t...Continue Reading

References

Mar 16, 2002·Toxicological Sciences : an Official Journal of the Society of Toxicology·Wout Slob
May 1, 1951·Journal of General Microbiology·D J FINNEY
Apr 7, 2009·Journal of Theoretical Biology·Gregory J Howard, Thomas F Webster
Jan 28, 2010·Analytica Chimica Acta·Sebastian BuchingerGeorg Reifferscheid
Sep 8, 2010·Environmental Toxicology and Chemistry·Christian Ritz
May 24, 2011·Regulatory Toxicology and Pharmacology : RTP·Gregg E Dinse, David M Umbach
Mar 17, 2012·Environmental Science and Pollution Research International·Sebastian SchmittSebastian Buchinger
Sep 20, 2012·Integrated Environmental Assessment and Management·John W GreenJane P Staveley
Oct 4, 2012·Journal of Environmental Monitoring : JEM·Beate I EscherMatti Lang
Dec 29, 2013·Environmental Science & Technology·Beate I EscherFrederic D L Leusch
May 31, 2014·Water Research·Janet Y M TangBeate I Escher
Oct 31, 2015·Environmental Science & Technology·Peta A NealeBeate I Escher
Apr 19, 2016·Environmental Toxicology and Chemistry·David R Fox, Wayne G Landis
May 22, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Richard JudsonRussell S Thomas
Oct 27, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Markus BrinkmannThomas G Preuss
Mar 2, 2018·Environmental Science. Processes & Impacts·Peta A NealeBeate I Escher
Jun 23, 2018·The Science of the Total Environment·Muhammad Arslan Kamal HashmiWerner Brack

❮ Previous
Next ❯

Citations

Oct 23, 2018·Environmental Science. Processes & Impacts·Annika JahnkeBeate I Escher
Oct 16, 2019·Environmental Toxicology and Chemistry·Muhammad Arslan Kamal HashmiWerner Brack
Jul 24, 2020·Environmental Health Perspectives·Beate I EscherFabian C Fischer
Feb 13, 2020·Nature Communications·Beate LeppertTobias Polte
Feb 23, 2020·Journal of Applied Toxicology : JAT·Masaru IharaHiroaki Tanaka
May 28, 2020·Analytical and Bioanalytical Chemistry·Alan J BergmannEtiënne L M Vermeirssen
Jan 25, 2020·Science·Beate I EscherEmma L Schymanski
Jun 24, 2019·Archives of Toxicology·Luise HennebergerBeate I Escher
Sep 9, 2020·Environmental Toxicology and Chemistry·Beate EscherChristiane Zarfl
Sep 15, 2020·Environmental Toxicology and Chemistry·Lisa Glauch, Beate I Escher
Dec 1, 2020·Environmental Toxicology and Chemistry·Beate I Escher, Peta A Neale
Nov 2, 2020·The Science of the Total Environment·Shangbo ZhouHenner Hollert
Mar 20, 2021·The Science of the Total Environment·Elizabeth Medlock KakaleyVickie S Wilson
May 7, 2021·The Science of the Total Environment·Livia GómezTeresa Lettieri
Jun 24, 2021·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Erica SelinJohan Lundqvist
Jul 18, 2019·Chemical Research in Toxicology·Beate I EscherRita Schlichting
Jul 23, 2019·Chemical Research in Toxicology·Fabian C FischerBeate I Escher
Nov 21, 2020·Environmental Science & Technology·Melis MuzAnnika Jahnke
May 24, 2019·Environmental Science & Technology·Johannes VölkerMartin Wagner
May 6, 2020·Chemical Research in Toxicology·Julia HuchthausenLuise Henneberger
Jul 2, 2019·Environmental Science & Technology·Christoph D RummelAnnika Jahnke
Nov 21, 2021·Archives of Toxicology·Erica SelinJohan Lundqvist

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.