PMID: 9434121Jan 20, 1998Paper

The AGDV residues on the gamma chain carboxyl terminus of platelet-bound fibrinogen are needed for platelet aggregation

Biochimica Et Biophysica Acta
Q LiuM M Frojmovic

Abstract

It has been clear that only the carboxyl terminus of fibrinogen (Fg) gamma chain is required for the initial binding of Fg from solution to its GPIIbIIIa (glycoprotein IIb and IIIa) receptor on activated platelets, whereas the two RGD sites on the A alpha chain do not play any role. In this study, we examined the role of these three putative adhesive domains on Fg already bound to its receptors in mediating platelet aggregation. Activated platelets were first incubated with Fg to let the Fg bind, then with monoclonal antibodies (mAb) to block the putative adhesive domains, and the platelet suspension was then sheared or stirred to induce aggregation. The mAb 4A5, which recognizes the last four amino acid residues (AGDV) in a dodecapeptide (H12) on the carboxyl terminus of the Fg gamma chain, markedly inhibited platelet aggregation. Z69/8, a mAb whose epitope is also on the dodecapeptide but does not recognize the AGDV residues, did not have any inhibitory effect on aggregation. The anti-RGDS and anti-RGDF mAbs did not affect both macro- and micro-aggregation at all, whether tested singly or together. These results demonstrate that, similar to the situation for the initial binding of soluble Fg, only the gamma chain carboxyl ter...Continue Reading

References

Nov 1, 1979·The Journal of Clinical Investigation·J S Bennett, G Vilaire
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D H FarrellE W Davie
Nov 1, 1991·Journal of Leukocyte Biology·Y P Rochon, M M Frojmovic
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Oct 1, 1994·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·T K GartnerJ M Derrick

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Citations

Mar 19, 2013·Journal of Biomedical Materials Research. Part a·Elizabeth M Srokowski, Kimberly A Woodhouse
May 29, 2000·Biophysical Journal·A BonnefoyM M Frojmovic
Feb 22, 2000·The Journal of Biological Chemistry·S R Adderley, D J Fitzgerald

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