The agonist-induced phosphorylation of the rat follitropin receptor maps to the first and third intracellular loops

Molecular Endocrinology
K NakamuraMario Ascoli

Abstract

Previous results from this laboratory have shown that the rat FSH receptor (rFSHR) becomes phosphorylated on S/T residues upon stimulation of transfected cells with human (h)FSH and that a truncation of the C-terminal tail that removes 12 of the 25 intracellular S/T residues does not affect phosphorylation. Based on the results of phosphopeptide-mapping experiments we analyzed three new mutants. rFSHR-1L and rFSHR-3L were constructed by mutating the S/T residues in the first intracellular loop or the third intracellular loop, respectively. rFSHR-(3L+CT) was constructed by mutating all the S/T residues in the third loop as well as S624, the only C-terminal tail residue that was not previously eliminated as a potential phosphorylation site. All mutants were biologically active. The agonist-induced phosphorylation of rFSHR-3L and rFSHR-(3L+CT) were partially reduced, while that of rFSHR-1L was almost completely lost. The agonist-induced uncoupling of rFSHR-1L and rFSHR-3L are retarded to about the same extent, while the agonist-induced internalization is retarded only in rFSHR-1L. Four major conclusions can be made from the present studies: 1) the phosphorylated rFSHR is a common molecular intermediate in agonist-induced uncouplin...Continue Reading

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Citations

Aug 4, 2015·Frontiers in Endocrinology·Antara A Banerjee, Smita D Mahale
Mar 26, 2015·Frontiers in Pharmacology·Sabrina M de MunnikHenry F Vischer
Dec 2, 1999·Japanese Journal of Pharmacology·C AkiyamaH Kurose
Jan 15, 2008·British Journal of Pharmacology·A B Tobin
Feb 5, 2008·Endocrine·Alfredo Ulloa-AguirreJames A Dias
Aug 9, 2003·Molecular Endocrinology·Hanumanthappa KrishnamurthyMario Ascoli
Jun 19, 2001·American Journal of Physiology. Endocrinology and Metabolism·F ClémentD Claude
May 27, 2021·Biological Chemistry·Lizzy WankaAnnette G Beck-Sickinger
Oct 19, 2006·Molecular and Cellular Endocrinology·Alfredo Ulloa-AguirreJames A Dias

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