The Agrobacterium vitis T-6b oncoprotein induces auxin-independent cell expansion in tobacco

The Plant Journal : for Cell and Molecular Biology
Bernadette ClémentL Otten

Abstract

Among the Agrobacterium T-DNA genes, rolB, rolC, orf13, orf8, lso, 6b and several other genes encode weakly homologous proteins with remarkable effects on plant growth. The 6b oncogene induces tumors and enations. In order to study its properties we have used transgenic tobacco plants that carry a dexamethasone-inducible 6b gene, dex-T-6b. Upon induction, dex-T-6b plants develop a large array of morphological modifications, some of which involve abnormal cell expansion. In the present investigation, dex-T-6b-induced expansion was studied in intact leaves and an in vitro leaf disc system. Although T-6b and indole-3-acetic acid (IAA) both induced expansion and were non-additive, T-6b expression did not increase IAA levels, nor did it induce an IAA-responsive gene. Fusicoccin (FC) is known to stimulate expansion by increasing cell wall plasticity. T-6b- and FC-induced expansion were additive at saturating FC concentrations, indicating that T-6b does not act by a similar mechanism to FC. T-6b expression led to higher leaf osmolality values, in contrast to FC, suggesting that the T-6b gene induces expansion by increasing osmolyte concentrations. Metabolite profiling showed that glucose and fructose played a major role in this increa...Continue Reading

References

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Citations

Jan 30, 2008·Pflügers Archiv : European journal of physiology·Geoffrey Duby, Marc Boutry
Mar 3, 2007·Planta·Sasan AmirsadeghiGreg C Vanlerberghe
Feb 9, 2012·Molecular Genetics and Genomics : MGG·Jian WuGang Lu
Sep 25, 2007·The Plant Cell·Shinji TerakuraYasunori Machida
Nov 29, 2011·Yi chuan = Hereditas·Yun-Kai JinYing Ruan
Apr 12, 2016·Frontiers in Plant Science·Grace ArmijoPatricio Arce-Johnson
Feb 20, 2015·Journal of Plant Research·Masaki Ito, Yasunori Machida
Oct 30, 2019·The Plant Journal : for Cell and Molecular Biology·Thomas PotuschakLéon Otten

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