The Ah receptor recognizes DNA binding sites for the B cell transcription factor, BSAP: a possible mechanism for dioxin-mediated alteration of CD19 gene expression in human B lymphocytes

Biochemical and Biophysical Research Communications
S A Masten, K T Shiverick

Abstract

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) inhibits murine and human B lymphocyte immunoglobulin production through an unknown mechanism. This study investigated the effect of TCDD on expression of the CD19 gene in a human B lymphocyte cell line. Northern blot analysis showed that TCDD treatment decreased steady state levels of CD19 mRNA by 67% in the IM-9 cell line. Using a gel mobility shift assay, we identified a DNA-binding complex in IM-9 nuclear extracts that by several criteria appears to be the Ah receptor. In addition, the Ah receptor complex recognized a DNA binding site for B cell lineage-specific activator protein (BSAP) in the promoter region of the human CD19 gene which is similar to the consensus Ah receptor DNA binding site. These results suggest that the AhR could interfere with BSAP-stimulated CD19 gene transcription by competition for a common DNA binding site.

Citations

Oct 19, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Courtney E W Sulentic, Norbert E Kaminski
Mar 26, 2010·Environmental Health : a Global Access Science Source·Lenka L Allan, David H Sherr
Feb 27, 2003·Biochemical and Biophysical Research Communications·Patrik AnderssonAnnika Hanberg
Aug 24, 2000·Critical Reviews in Toxicology·J J WhyteD E Tillitt
Oct 6, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jinpeng LiNorbert E Kaminski
Oct 30, 2008·Journal of Immunotoxicology·Alessandra GennariLaura Gribaldo
Jan 6, 2005·Springer Seminars in Immunopathology·Eric C B MilnerIñaki Sanz
Feb 24, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tharu M FernandoCourtney E W Sulentic
Nov 16, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Anahita BhathenaDavid S Riddick
Feb 12, 2011·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Amjad MustafaRobert Gogal

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