The alkali molten globule state of ferrocytochrome c: extraordinary stability, persistent structure, and constrained overall dynamics

Biochemistry
D Krishna RaoAbani K Bhuyan

Abstract

This paper describes the structural and dynamic properties of a hitherto uncovered alkali molten globule (MG) state of horse "ferrocytochrome c" (ferrocyt c). Several experimental difficulties mainly because of heme autoxidation and extraordinary stability of ferrocyt c have been overcome by working with the carbonmonoxide-bound molecule under extremely basic condition (pH 13) in a strictly anaerobic atmosphere. Structural and molecular properties extracted from basic spectroscopic experiments suggest that cations drive the base-denatured CO-liganded protein to the MG state. The stability of this state is approximately 5.2 kcal mol(-)(1), and the guanidinium-induced unfolding transition is sharp (m(g) approximately 2.3 kcal mol(-)(1) M(-)(1)), suggesting contents of rigid tertiary structure. Strategic experiments involving the measurement of the CO association rate to the base-denatured protein and intrachain diffusion rates measured by laser photolysis of CO indicate a substantially restricted overall motion and stiffness of the polypeptide chain in the MG state. Possible placement of the state in the folding coordinate of ferrocyt c is discussed.

References

Dec 7, 1992·FEBS Letters·V N UverskyO B Ptitsyn
Dec 5, 1991·Journal of Molecular Biology·Y Goto, S Nishikiori
Jan 1, 1985·European Biophysics Journal : EBJ·D A DolgikhO B Ptitsyn
Jul 5, 1974·Journal of Molecular Biology·P L Privalov, N N Khechinashvili
Dec 28, 1981·FEBS Letters·D A DolgikhO B Ptitsyn
Mar 14, 1994·FEBS Letters·O B Ptitsyn, V N Uversky
Feb 5, 1993·Journal of Molecular Biology·M KataokaY Goto
May 20, 1993·Journal of Molecular Biology·Y HagiharaY Goto
Oct 3, 2001·Journal of Molecular Biology·A K Bhuyan, J B Udgaonkar
May 23, 2003·Journal of Biochemistry·A A Moosavi-MovahediG H Hakimelahi
Mar 6, 2004·Journal of Molecular Biology·N Prakash PrabhuAbani K Bhuyan

❮ Previous
Next ❯

Citations

Mar 11, 2011·Biochemistry·Shigeyoshi NakamuraShun-ichi Kidokoro
Nov 20, 2012·Journal of Biochemistry·Rishu JainRajesh Kumar
Mar 25, 2011·Metallomics : Integrated Biometal Science·Md Khurshid Alam KhanFaizan Ahmad
Sep 20, 2015·Archives of Biochemistry and Biophysics·Rishu JainRajesh Kumar
Oct 6, 2009·Biopolymers·Abani K Bhuyan
Oct 10, 2006·Journal of Molecular Biology·Rajesh KumarAbani K Bhuyan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.