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The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology

ACS Synthetic Biology

Jan 29, 2016

Jonathan GaramellaVincent Noireaux

Abstract

We report on and provide a detailed characterization of the performance and properties of a recently developed, all Escherichia coli, cell-free transcription and translation system. Gene expression is entirely based on the endogenous translation components and transcription machinery pr...read more

Mentioned in this Paper

Metabolic Process, Cellular
ATP Regeneration
Solocyte
Protein Degradation, Regulatory
MazF protein, Mycobacterium tuberculosis
Alkalescens-Dispar Group
Testolactone
Transcription, Genetic
Peptide Hydrolases
Bacteriophages
20
390
Paper Details
References
  • References77
  • Citations34
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  • References77
  • Citations34
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The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology

ACS Synthetic Biology

Jan 29, 2016

Jonathan GaramellaVincent Noireaux

PMID: 26818434

DOI: 10.1021/acssynbio.5b00296

Abstract

We report on and provide a detailed characterization of the performance and properties of a recently developed, all Escherichia coli, cell-free transcription and translation system. Gene expression is entirely based on the endogenous translation components and transcription machinery pr...read more

Mentioned in this Paper

Metabolic Process, Cellular
ATP Regeneration
Solocyte
Protein Degradation, Regulatory
MazF protein, Mycobacterium tuberculosis
Alkalescens-Dispar Group
Testolactone
Transcription, Genetic
Peptide Hydrolases
Bacteriophages
20
390

Similar Papers Found In These Feeds

Artificial Cell Models

This feed focuses on biomimetrics, synthetic biology and bio- and tissue-engineering approaches used for modeling human diseases.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

ACS Synthetic Biology

An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells

ACS Synthetic BiologyJanuary 20, 2012
Jonghyeon Shin, Vincent Noireaux
Journal of Visualized Experiments : JoVE

Protocols for implementing an Escherichia coli based TX-TL cell-free expression system for synthetic biology

Journal of Visualized Experiments : JoVEOctober 3, 2013
Zachary Z SunVincent Noireaux
Paper Details
References
  • References77
  • Citations34
12345...
  • References77
  • Citations34
1234
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