The allosteric behavior of Fur mediates oxidative stress signal transduction in Helicobacter pylori

Frontiers in Microbiology
Simone PelliciariAlberto Danielli

Abstract

The microaerophilic gastric pathogen Helicobacter pylori is exposed to oxidative stress originating from the aerobic environment, the oxidative burst of phagocytes and the formation of reactive oxygen species, catalyzed by iron excess. Accordingly, the expression of genes involved in oxidative stress defense have been repeatedly linked to the ferric uptake regulator Fur. Moreover, mutations in the Fur protein affect the resistance to metronidazole, likely due to loss-of-function in the regulation of genes involved in redox control. Although many advances in the molecular understanding of HpFur function were made, little is known about the mechanisms that enable Fur to mediate the responses to oxidative stress. Here we show that iron-inducible, apo-Fur repressed genes, such as pfr and hydA, are induced shortly after oxidative stress, while their oxidative induction is lost in a fur knockout strain. On the contrary, holo-Fur repressed genes, such as frpB1 and fecA1, vary modestly in response to oxidative stress. This indicates that the oxidative stress signal specifically targets apo-Fur repressed genes, rather than impairing indiscriminately the regulatory function of Fur. Footprinting analyses showed that the oxidative signal s...Continue Reading

References

Jan 6, 2000·Archives of Biochemistry and Biophysics·D Touati
Sep 17, 2005·Molecular Microbiology·Ge WangRobert J Maier
Nov 22, 2005·Nature Methods·Thomas J AlbertDouglas E Berg
Jun 13, 2006·The Journal of Biological Chemistry·Jin-Won Lee, John D Helmann
Jun 22, 2006·Journal of Bacteriology·Alberto DanielliVincenzo Scarlato
Aug 2, 2006·Molecular Microbiology·Ge WangRobert J Maier
Jan 12, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jin-Won Lee, John D Helmann
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·David R ScottGeorge Sachs
Apr 7, 2009·Journal of Bacteriology·Alberto DanielliVincenzo Scarlato
Apr 15, 2009·Infection and Immunity·Beth M CarpenterD Scott Merrell
Apr 29, 2009·PloS One·Beth M CarpenterD Scott Merrell
Jun 4, 2010·Antioxidants & Redox Signaling·Hitoshi TsugawaToshifumi Hibi
Jun 24, 2010·The Journal of Microbiology·Shana MilesD Scott Merrell
Jun 29, 2010·FEMS Microbiology Reviews·Alberto Danielli, Vincenzo Scarlato
Aug 24, 2011·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·John T LohTimothy L Cover
Jun 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·James ButcherAlain Stintzi
Jul 17, 2012·Journal of Bacteriology·James M Dubbs, Skorn Mongkolsuk
Sep 25, 2012·Journal of Bacteriology·Jeremy J GilbreathD Scott Merrell
May 9, 2013·Nature Reviews. Microbiology·Nina R SalamaAnne Müller
Oct 8, 2013·Journal of Bacteriology·Beth M CarpenterD Scott Merrell
Oct 10, 2013·Frontiers in Cellular and Infection Microbiology·Bryan Troxell, Hosni M Hassan
Sep 1, 2011·European Journal of Microbiology & Immunology·C BelzerE J Kuipers

❮ Previous
Next ❯

Citations

Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat
Aug 26, 2016·Nature Communications·Davide RoncaratiAlberto Danielli
Dec 22, 2019·The FEBS Journal·Roberta EspositoAlessandra Tosco
Mar 12, 2016·PloS One·Andrés GonzálezMaría F Fillat
Aug 2, 2018·Frontiers in Microbiology·Mauricio LatorreVerónica Cambiazo
Jun 18, 2021·Nucleic Acids Research·Piotr SzczepanowskiAnna Zawilak-Pawlik

❮ Previous
Next ❯

Methods Mentioned

BETA
Footprinting
PCR
ChIP-Chip

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.