The Allosteric Site for the Nascent Cell Wall in Penicillin-Binding Protein 2a: An Achilles' Heel of Methicillin-Resistant Staphylococcus aureus

Current Medicinal Chemistry
Iván AcebrónJuan A Hermoso

Abstract

The ability to resist the effect of a wide range of antibiotics makes methicillin-resistant Staphylococcus aureus (MRSA) a leading global human pathogen. A key determinant of resistance to β-lactam antibiotics in this organism is penicillin-binding protein 2a (PBP2a), an enzyme that catalyzes the crosslinking reaction between two adjacent peptide stems during the peptidoglycan biosynthesis. The recently published crystal structure of the complex of PBP2a with ceftaroline, a cephalosporin antibiotic that shows efficacy against MRSA, has revealed the allosteric site at 60-Å distance from the transpeptidase domain. Binding of ceftaroline to the allosteric site of PBP2a triggers conformational changes that lead to the opening of the active site from a closed conformation, where a second molecule of ceftaroline binds to give inhibition of the enzyme. The discovery of allostery in MRSA remains the only known example of such regulation of cellwall biosynthesis and represents a new paradigm in fighting MRSA. This review summarizes the present knowledge of the allosteric mechanism, the conformational changes allowing PBP2a catalysis and the means by which some clinical strains have acquired resistance to ceftaroline by disrupting the al...Continue Reading

Citations

Jun 22, 2016·Future Medicinal Chemistry·David Yuxin WangChristopher J Schofield
Mar 14, 2018·Microorganisms·Pietro SpezialeGiampiero Pietrocola
Nov 21, 2019·Protein Science : a Publication of the Protein Society·Jed F Fisher, Shahriar Mobashery
Oct 24, 2020·Scientific Reports·Tatiana TozarMihaela Oana Romanitan
Apr 29, 2020·Journal of Medicinal Chemistry·Yuanyuan QianMayland Chang

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