PMID: 8592140Feb 1, 1996Paper

The alpha 1 and alpha 6 subunits can coexist in the same cerebellar GABAA receptor maintaining their individual benzodiazepine-binding specificities

Journal of Neurochemistry
Z U KhanA L De Blas

Abstract

Two GABAA receptor subunit-specific antibodies anti-alpha 6 and anti-alpha 1 have been used for elucidating the relationship between the presence of alpha 1 and/or alpha 6 subunits in the cerebellar GABAA receptors and the benzodiazepine-binding specificity. Receptor immunoprecipitation with the subunit-specific antibodies shows that 39% of the cerebellar GABAA receptors have alpha 6, whereas 76% of the receptors have alpha 1 as determined by [3H]-muscimol binding. Results show that 42-45% of the receptors having alpha 6 also have alpha 1, whereas 13-15% of the receptors that contain alpha 1 also have alpha 6. The immunoprecipitation results as well as immunopurification and immunoblotting experiments reveal the existence of three types of cerebellar GABAA receptors; i.e., one has both alpha 1 and alpha 6 subunits, a second type has alpha 1 but not alpha 6, and a third type has alpha 6 but not alpha 1 subunits. The results also show that receptors where alpha 1 and alpha 6 subunits coexist have two pharmacologically different benzodiazepine-binding properties, each associated with a different alpha subunit. The alpha 1 subunit contributes the high-affinity binding of [3H]Ro 15-1788 (flumazenil) and the diazepam-sensitive bindin...Continue Reading

Citations

Feb 1, 1996·Molecular Neurobiology·A L De Blas
Feb 1, 1996·Neurochemical Research·Z U KhanA L De Blas
Dec 22, 1999·British Journal of Pharmacology·M AkiyamaS Shibata
Mar 11, 2011·Journal of Neurochemistry·Robert A Rissman, William C Mobley
Jan 17, 2014·The Journal of Clinical Investigation·Aline WinkelmannJochen C Meier
Jul 31, 2003·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Christian CaldjiMichael J Meaney
Sep 27, 2002·The Journal of Biological Chemistry·Sabine W BaumannErwin Sigel
Jun 27, 1997·The Journal of Biological Chemistry·M Li, A L De Blas
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Frédéric Minier, Erwin Sigel
Jul 22, 1999·Annals of the New York Academy of Sciences·P J WhitingK A Wafford
Dec 26, 2001·The Journal of Pharmacology and Experimental Therapeutics·H MöhlerU Rudolph
Sep 22, 2009·Journal of Neurochemistry·Roland BaurErwin Sigel
Aug 24, 2007·Journal of Neurochemistry·Robert A RissmanDavid M Armstrong
Oct 31, 2020·Frontiers in Synaptic Neuroscience·Petra ScholzeMargot Ernst
Apr 1, 1997·Brain Research. Molecular Brain Research·A GutiérrezA L De Blas
Jan 1, 1996·Neuropharmacology·W WisdenS Bahn
Jan 1, 1996·Neuropharmacology·Z U KhanA L De Blas
Apr 21, 1999·Brain Research. Brain Research Reviews·A K Mehta, M K Ticku
Sep 29, 2004·Biochemical Pharmacology·Stephen L BoehmR Adron Harris
May 11, 2011·Neuron·Bernhard LuscherCasey L Kilpatrick

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