PMID: 9177167Jun 10, 1997Paper

The alpha-bungarotoxin binding site on the nicotinic acetylcholine receptor: analysis using a phage-epitope library

Proceedings of the National Academy of Sciences of the United States of America
M BalassS Fuchs

Abstract

The nicotinic acetylcholine receptor (AcChoR) is a ligand-gated ion channel that is activated upon binding of acetylcholine. alpha-Neurotoxins, in particular alpha-bungarotoxin (alpha-BTX), bind specifically and with high affinity to the AcChoR and compete with binding of the natural ligand. We employed a 15-mer phage-display peptide library to select epitopes reacting with alpha-BTX. Phages bearing the motif YYXSSL as a consensus sequence were found to bind with high affinity to alpha-BTX. The library-derived peptide (MRYYESSLKSYPD) bears amino acid sequence similarities to a region of the alpha-subunit of the Torpedo muscle AcChoR, as well as of other muscle and neuronal AcChoRs that bind alpha-BTX. The library-derived peptide and the corresponding peptides containing residues 187-199 of the Torpedo AcChoR alpha-subunit (WVYYTCCPDTPYL), as well as peptides analogous to the above region in the neuronal AcChoR (e.g., human alpha7; ERFYECCKEPYPD) that binds alpha-BTX, inhibit the binding of alpha-BTX to the intact Torpedo AcChoR with IC50 values of 10(-6) M. A synthetic peptide from a neuronal AcChoR that does not bind alpha-BTX (e.g., human alpha2; ERKYECCKEPYPD) which differs by just one amino acid from the homologous peptide ...Continue Reading

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Citations

Jan 16, 2002·Journal of Receptor and Signal Transduction Research·M Dani
Feb 3, 2004·Journal of Receptor and Signal Transduction Research·Michael Szardenings
Apr 8, 2010·Current Protocols in Neuroscience·Andrew R M Bradbury
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·T ScherfJ Anglister
Jul 21, 1998·Annals of the New York Academy of Sciences·S ArielS Fuchs
Nov 1, 2003·Annals of the New York Academy of Sciences·Sara FuchsEphraim Katchalski-Katzir
Jan 16, 2002·Journal of Receptor and Signal Transduction Research·M Dani
Apr 23, 2008·Current Protocols in Neuroscience·A Bradbury
Jun 30, 2016·Scientific Reports·Björn BeckerManfred J Schmitt
Apr 5, 2020·Drug Testing and Analysis·Mark TimmsRohan Steel
Jun 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·T ScherfE Katchalski-Katzir
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Corey M McCannJoshua R Sanes
Jan 28, 2004·Journal of Pharmacological Sciences·Selvanayagam Nirthanan, Matthew C E Gwee
Jul 27, 2007·The FEBS Journal·Dimitra KalamidaSocrates J Tzartos
Jul 16, 2005·BioTechniques·Corey M McCannJoshua R Sanes
Mar 17, 2001·Chemistry & Biology·R KasherE Katchalski-Katzir
May 12, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Woo-Hyeon Byeon, Bernard Weisblum
Feb 13, 2009·Theoretical Biology & Medical Modelling·Adak NasiripourdoriHossein Naderi-Manesh

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