The alpha-factor mating pheromone of Saccharomyces cerevisiae: a model for studying the interaction of peptide hormones and G protein-coupled receptors

Peptides
Fred Naider, Jeffrey M Becker

Abstract

Mating in Saccharomyces cerevisiae is initiated by the secretion of diffusible peptide pheromones that are recognized by G protein-coupled receptors (GPCR). This review summarizes the use of the alpha-factor (WHWLQLKPGQPMY)--GPCR (Ste2p) interaction as a paradigm to understand the recognition between medium-sized peptide hormones and their cognate receptors. Studies over the past 15 years have indicated that the alpha-factor is bent around the center of the pheromone and that residues near the amine terminus play a central role in triggering signal transduction. The bend in the center appears not to be rigid and this flexibility is likely necessary for conformational changes that occur as the receptor switches from the inactive to active state. The results of synthetic, biological, biochemical, molecular biological, and biophysical analyses have led to a preliminary model for the structure of the peptide bound to its receptor. Antagonists for Ste2p have changes near the N-terminus of alpha-factor, and mutated forms of Ste2p were discovered that appear to favor binding of these antagonists relative to agonists. Many features of this yeast recognition system are relevant to and have counterparts in mammalian cells.

References

Jun 1, 1979·Biophysical Journal·P Y Chou, G D Fasman
Oct 10, 1977·Biochemical and Biophysical Research Communications·E CiejekM Geier
Feb 28, 1979·Biochemical and Biophysical Research Communications·Y MasuiS Sakakibara
Feb 1, 1979·The Journal of Cell Biology·J S Tkacz, V L MacKay
Aug 1, 1992·Antonie van Leeuwenhoek·J B Konopka, S Fields
Jan 1, 1992·Annual Review of Biochemistry·J Rizo, L M Gierasch
Jan 1, 1991·Annual Review of Biochemistry·H G DohlmanR J Lefkowitz
Jan 1, 1991·Annual Review of Cell Biology·L MarshI Herskowitz
Jan 1, 1991·Enzyme·Y BourbonnaisD Y Thomas
Jun 1, 1991·Biopolymers·C García-EcheverríaM Pons
May 1, 1991·International Journal of Peptide and Protein Research·F AlbericioG Barany
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K J Blumer, J Thorner
Mar 31, 1989·Biochemical and Biophysical Research Communications·A S DuttaJ R Woodburn
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L Marsh, I Herskowitz
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D F Sargent, R Schwyzer
Dec 9, 1985·Nucleic Acids Research·A C Burkholder, L H Hartwell
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D C HagenG F Sprague
Oct 7, 1986·Biochemistry·R Schwyzer
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·V L MacKayM L Parker
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R W BehlingL W Jelinski
Jan 1, 1986·CRC Critical Reviews in Biochemistry·F Naider, J M Becker
Aug 11, 1987·Nucleic Acids Research·M Egel-Mitani, M T Hansen
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C M Deber, B A Behnam

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Citations

Jan 20, 2009·Biochemistry·George K E UmanahJeffrey M Becker
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kevin Alby, Richard J Bennett
Oct 15, 2011·Molecular Biology of the Cell·Ching-Hsuan LinRichard J Bennett
Apr 15, 2011·FEMS Yeast Research·Jürgen WendlandAndrea Walther
Aug 31, 2012·Microbiology and Molecular Biology Reviews : MMBR·Susan Michaelis, Jemima Barrowman
May 18, 2007·Annual Review of Microbiology·Malcolm Whiteway, Catherine Bachewich
May 15, 2010·Cell Division·Jorrit M Enserink, Richard D Kolodner
Oct 11, 2008·PLoS Computational Biology·Marcelo BeharTimothy C Elston
Jan 22, 2011·Molecular Biology and Evolution·Simon H MartinEmma T Steenkamp
Feb 4, 2009·The Journal of Cell Biology·Casey A Ydenberg, Mark D Rose
Feb 13, 2016·Fungal Genetics and Biology : FG & B·Birgit Wiltschi
Apr 19, 2011·Fungal Genetics and Biology : FG & B·Stephen K Jones, Richard J Bennett
May 4, 2005·Yeast
Aug 29, 2006·Angewandte Chemie·Laurie L ParkerStephen J Kron
Sep 24, 2013·Biochimica Et Biophysica Acta·Rajashri SridharanMark E Dumont
Jul 4, 2008·Acta Microbiologica Et Immunologica Hungarica·Erika Kothe
Aug 14, 2012·Fungal Genetics and Biology : FG & B·Christian SeibelMonika Schmoll
Apr 17, 2007·Biochimica Et Biophysica Acta·Anshika BajajMark E Dumont
Apr 25, 2006·FEMS Yeast Research·Roberto CoriaGerardo Coello
May 18, 2007·FEMS Yeast Research·Laura Ongay-LariosRoberto Coria
May 14, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Haiyu YuanChunxiong Luo
Nov 24, 2017·Scientific Reports·Raphaël B Di RobertoSergio G Peisajovich
Jul 19, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Shalini GourJay Kant Yadav
Jan 20, 2017·The Journal of Biological Chemistry·Stefania VitaleMarta Bruix
Apr 11, 2019·Current Genetics·Taisuke Seike
Sep 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fred Naider, Jeffrey M Becker
Jul 3, 2018·Frontiers in Microbiology·Vladimir J ArellanoGabriel Del Rio

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