The alpha-L-Rhamnose recognizing lectin site of human dermal fibroblasts functions as a signal transducer: modulation of Ca2+ fluxes and gene expression

Biochimica Et Biophysica Acta
G FauryL Robert

Abstract

An alpha-l-Rhamnose specific lectin site was described on human skin keratinocytes and fibrobasts. The addition of Rhamnose-rich oligo- and polysaccharides (RROPs) to fibroblasts has been shown to stimulate cell proliferation and increase extracellular matrix biosynthesis, suggesting that this lectin site functions as a "true" receptor transmitting messages to the cell interior. It was confirmed here that addition of the Rhamnose-rich polysaccharide, RROP-1, to normal human dermal fibroblasts (NHDFs) and human endothelial cells produced a dose-dependent stimulation of the calcium-signaling pathway, inducing fast and transient increases in Ca2+ influx and intracellular free Ca2+ level. The Rhamnose-rich oligosaccharide RROP-3 as well as l-Rhamnose alone were also able to trigger similar intracellular free Ca2+ concentration increases in NHDFs. Moreover, the recording of the RROP-1-induced modification of the gene-expression profile in fibroblasts showed that this polysaccharide triggered a down-regulation of the expression of several growth factors, adhesion molecules and extracellular matrix proteins involved in pro-tumoral activity and/or fibrotic processes. These results further support the hypothesis of a receptor function f...Continue Reading

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Citations

Feb 4, 2014·Developmental and Comparative Immunology·Matteo CammarataNicolò Parrinello
Feb 20, 2014·International Journal of Biological Macromolecules·Yu-Ming WangChang-Hu Xue
Sep 29, 2011·Chembiochem : a European Journal of Chemical Biology·Yanyan WangHongxiang Lou
Jul 15, 2015·Zygote : the Biology of Gametes and Early Embryos·Jing LiangSteven B Oppenheimer
Apr 7, 2017·Phytotherapy Research : PTR·Fatemeh Kalalinia, Iman Karimi-Sani
Mar 8, 2019·International Journal of Cosmetic Science·H PageonD Asselineau

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