The alpha-subunit of the mammalian guanine nucleotide-exchange factor eIF-2B is essential for catalytic activity in vitro

Biochemical and Biophysical Research Communications
B L Craddock, C G Proud

Abstract

Eukaryotic initiation factor (eIF)-2B, the guanine nucleotide exchange factor for eIF-2, consists of five distinct subunits in both mammals and the yeast Saccharomyces cerevisiae. The exchange reaction mediated by eIF-2B can be regulated by phosphorylation of eIF-2 on its alpha-subunit. This represents a key control point in the initiation of translation. The functions of the individual subunits of the eIF-2B complex remain unclear. Mutational analysis in Saccharomyces cerevisiae suggested that the smallest subunit (the alpha) is dispensable for exchange, but required for the inhibition of eIF-2B by eIF-2(alphaP). Here we present evidence that, in mammalian cells, eIF-2Balpha is essential for the activity of the complex, since preparations of eIF-2B lacking this subunit are not active in nucleotide exchange in vitro, although the complex still contains the beta, gamma, delta and epsilon subunits.

Citations

Jan 13, 2012·The Journal of Biological Chemistry·Xuemin WangChristopher G Proud
Nov 29, 2015·The Biochemical Journal·Noel C WorthamChristopher G Proud
Feb 18, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Noel C WorthamChristopher G Proud
Apr 27, 2001·The Journal of Biological Chemistry·D D WilliamsC G Proud

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