The alpha(1D)-adrenergic receptor directly regulates arterial blood pressure via vasoconstriction

The Journal of Clinical Investigation
A TanoueG Tsujimoto

Abstract

To investigate the physiological role of the alpha(1D)-adrenergic receptor (alpha(1D)-AR) subtype, we created mice lacking the alpha(1D)-AR (alpha(1D)(-/-)) by gene targeting and characterized their cardiovascular function. In alpha(1D)-/- mice, the RT-PCR did not detect any transcript of the alpha(1D)-AR in any tissue examined, and there was no apparent upregulation of other alpha(1)-AR subtypes. Radioligand binding studies showed that alpha(1)-AR binding capacity in the aorta was lost, while that in the heart was unaltered in alpha(1D)-/- mice. Non-anesthetized alpha(1D)-/- mice maintained significantly lower basal systolic and mean arterial blood pressure conditions, relative to wild-type mice, and they showed no significant change in heart rate or in cardiac function, as assessed by echocardiogram. Besides hypotension, the pressor responses to phenylephrine and norepinephrine were decreased by 30-40% in alpha(1D)-/- mice. Furthermore, the contractile response of the aorta and the pressor response of isolated perfused mesenteric arterial beds to alpha(1)-AR stimulation were markedly reduced in alpha(1D)-/- mice. We conclude that the alpha(1D)-AR participates directly in sympathetic regulation of systemic blood pressure by va...Continue Reading

References

Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S CotecchiaB K Kobilka
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A MilanoR J Lefkowitz
Sep 15, 1993·Biochemical and Biophysical Research Communications·A HirasawaG Tsujimoto
Sep 1, 1996·British Journal of Pharmacology·X F DengD R Varma
Aug 1, 1996·Cellular Signalling·R D GuarinoM T Piascik
Mar 19, 1997·European Journal of Pharmacology·M IbarraR Villalobos-Molina
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·A CavalliS Cotecchia
Jun 8, 1999·The Journal of Biological Chemistry·A J ChruscinskiB K Kobilka
Jun 9, 2000·Annual Review of Physiology·W J KochH A Rockman
May 5, 2001·Pharmacology & Therapeutics·G A MichelottiD A Schwinn

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Citations

Aug 10, 2006·Cell and Tissue Research·Lutz Hein
Jun 16, 2004·Behavioural Brain Research·Kenichi MishimaMichihiro Fujiwara
Sep 7, 2002·Life Sciences·Akito TanoueGozoh Tsujimoto
Apr 3, 2003·Trends in Endocrinology and Metabolism : TEM·Akito TanoueGozoh Tsujimoto
May 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Taka-aki KoshimizuGozoh Tsujimoto
Dec 1, 2010·Proceedings of the National Academy of Sciences of the United States of America·John S LyssandChris Hague
May 13, 2008·The Journal of Biological Chemistry·John S LyssandChris Hague
Aug 30, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Heather Irina CohnAndrea D Eckhart
Jun 24, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Christopher J ClarkeJacqueline Ohanian
Nov 19, 2009·Circulation. Heart Failure·Brian C JensenPaul C Simpson
May 9, 2002·Biological & Pharmaceutical Bulletin·Taka-aki KoshimizuGozoh Tsujimoto
May 29, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Tobias BöhmerAdrian Gericke
Jan 20, 2004·Pharmacology & Therapeutics·Melanie Philipp, Lutz Hein
Aug 4, 2007·The Journal of Physiology·Yoko FujiwaraAkito Tanoue
Sep 29, 2011·Autonomic & Autacoid Pharmacology·M L Lázaro-SuárezR Villalobos-Molina
Nov 23, 2006·British Journal of Pharmacology·J S MortonJ C McGrath
Sep 20, 2005·British Journal of Pharmacology·Joseph ZachariaAllan MacDonald

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Methods Mentioned

BETA
genotyping
PCR
protein assay
competition

Software Mentioned

LIGAND

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