The alpha2beta1 integrin mediates the malignant phenotype on type I collagen in pancreatic cancer cell lines.

British Journal of Cancer
J J Grzesiak, Michael Bouvet

Abstract

Pancreatic cancer is characterised by a hallmark desmoplastic response that includes upregulated expression of the extracellular matrix, and type I collagen in particular. Recent studies indicate that pancreatic cancer cells stimulate type I collagen synthesis in adjacent stellate cells, and that this upregulated type I collagen expression promotes the malignant phenotype in tumour cells as defined by increased proliferation, resistance to chemically induced apoptosis, and increased tumorigenesis. The integrin specificity of this interaction between type I collagen and tumour cells was not identified, however. In the present study, we examined eight pancreatic cancer cell lines for adhesion, proliferation, and migration, on types I and IV collagen, fibronectin, laminin, and vitronectin, as well as integrin expression. Our results indicate, for the overwhelming majority of cell lines, that type I collagen promotes the strongest adhesion, proliferation, and migration relative to the other substrates tested. Utilising function-blocking monoclonal antibodies directed against particular integrin subunits in cell adhesion and migration inhibition assays, we demonstrate further that the malignant phenotype on type I collagen is mediat...Continue Reading

References

Nov 11, 1992·International Journal of Cancer. Journal International Du Cancer·R J WeinelS Santoso
Jan 1, 1991·The Journal of Clinical Investigation·E Ruoslahti
Jan 1, 1990·The Journal of Surgical Research·M P VezeridisP Calabresi
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Dec 1, 1988·Nature·A SonnenbergF Hogervorst
Jan 1, 1982·Methods in Enzymology·E RuoslahtiE Engvall
Jan 1, 1994·British Journal of Cancer·M LöhrG Klöppel
Jan 30, 2002·Journal of Surgical Oncology·Adrien A Tempia-CalieraMarkus W Büchler
Dec 3, 2002·Nature Reviews. Cancer·Nabeel Bardeesy, Ronald A DePinho
Mar 8, 2003·International Journal of Gastrointestinal Cancer·Andre Menke, Guido Adler
Mar 26, 2003·The American Journal of Pathology·Christine A Iacobuzio-DonahueMichael Goggins
Apr 15, 2003·Virchows Archiv : an International Journal of Pathology·Bence SiposGünter Klöppel
Nov 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Thomas ArmstrongJohn P Iredale
Dec 21, 2004·International Journal of Cancer. Journal International Du Cancer·John J GrzesiakMichael Bouvet
Mar 5, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Kohei MiuraBosco M C Chan

❮ Previous
Next ❯

Citations

Feb 5, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Annarosa ArcangeliLapo Bencini
Jul 31, 2008·The Journal of Biological Chemistry·Susan C Kirkland, Huijun Ying
Mar 12, 2009·Molecular Cancer Research : MCR·Nandor GaramszegiSean P Scully
Oct 27, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mikel Garcia-MarcosPradipta Ghosh
Mar 3, 2018·Expert Review of Gastroenterology & Hepatology·Shin Hamada, Atsushi Masamune
Dec 6, 2017·Advanced Healthcare Materials·Pradip Shahi ThakuriHossein Tavana
Oct 5, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Brigitta Matta-DomjanEirini G Velliou
Apr 14, 2011·Molecular Imaging·Chiun-Wei HuangPeter S Conti
Dec 20, 2007·Pancreas·John J GrzesiakMichael Bouvet
Oct 15, 2009·Journal of Experimental & Clinical Cancer Research : CR·Naomi WalshNorma O'Donovan
Jan 9, 2009·Pathology Oncology Research : POR·Teruhisa OharaKunio Yoshizawa
Dec 18, 2012·Clinical & Experimental Metastasis·Joseph L SottnikChristopher L Hall
Aug 30, 2014·World Journal of Gastroenterology : WJG·Zhihong XuMinoti V Apte
Jun 8, 2011·Molecular Carcinogenesis·Cui LiBin Shan
Mar 29, 2014·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Hiroaki MotegiKiyohiro Houkin
Feb 11, 2012·International Journal of Cancer. Journal International Du Cancer·Hiroaki KimuraRobert M Hoffman
Apr 26, 2020·Cells·Michele Sommariva, Nicoletta Gagliano
Aug 7, 2019·Current Topics in Medicinal Chemistry·Leonel Montealegre-SánchezEliécer Jiménez-Charris
Jun 12, 2013·International Journal of Cancer. Journal International Du Cancer·Prakash RadhakrishnanMichael A Hollingsworth
Apr 15, 2011·International Journal of Cancer. Journal International Du Cancer·John J GrzesiakMichael Bouvet

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
FACS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

© 2022 Meta ULC. All rights reserved