The alpha4 and alpha7 subunits and assembly of the 20S proteasome

FEBS Letters
Géraud-Sébastien ApcherR John Mayer

Abstract

The detailed mechanism of eukaryotic 20S proteasome assembly is currently unknown. In the present study, we demonstrate that the 20S proteasome subunits alpha4 and alpha7 interact with each other as well as all the alpha-subunits in vivo and in vitro. The N-terminal parts of alpha4 and alpha7 are essential for these newly discovered interactions in vitro. Glycerol gradient centrifugation of soluble extracts of HEK293 cells and Western blot analyses show that several alpha-subunits are found in non-proteasomal low-density fractions. The alpha4 and alpha7 subunits co-immunoprecipitate together from these low-density fractions. The unexpected interaction between alpha4 and alpha7 may provide a molecular basis for the formation of previously reported 13S and 16S assembly intermediates.

References

Nov 17, 1995·The Journal of Biological Chemistry·Y YangP A Peterson
Nov 1, 1994·Nature Structural Biology·P ZwicklW Baumeister
Oct 7, 1994·Cell·A Ciechanover
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·A LupasW Baumeister
Dec 31, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M Schmidt, P M Kloetzel
Feb 6, 1998·Journal of Molecular Biology·W L GerardsW Boelens
Jun 11, 1999·Molecular Biology Reports·L Jørgensen, K B Hendil
Nov 4, 2000·Nature Structural Biology·M GrollD Finley
May 17, 2002·Structure·Masaki UnnoTomitake Tsukihara
Jan 22, 2004·Biochemical and Biophysical Research Communications·Krupakar Jayarapu, Thomas A Griffin
Jan 24, 2004·The EMBO Journal·Irina VelichutinaMark Hochstrasser

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Citations

May 18, 2007·Biochemical and Biophysical Research Communications·Krupakar Jayarapu, Thomas A Griffin
Apr 30, 2010·Trends in Cell Biology·Lynn BedfordJeroen Roelofs
Apr 19, 2008·Reproductive Sciences·Bum-Yong KangHelen H Kay
Mar 17, 2010·Antiviral Chemistry & Chemotherapy·Saneyuki UjinoHiroshi Takaku
Jan 11, 2008·Proteomics·Chuan ChenTao Tao
Jul 16, 2016·The Journal of Biological Chemistry·Francis Wang DangKiran Madura

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