The alternative pathway C20 Delta8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5-fusion desaturase

FEBS Letters
O SayanovaJ A Napier

Abstract

A cDNA encoding a C20 Delta8-desaturase was isolated from the free-living soil amoeba, Acanthamoeba castellanii and functionally characterised by heterologous expression. The open reading frame of the A. castellanii C20 Delta8-desaturase showed similarity to other microsomal front-end desaturases, but the N-terminal domain contained a variant form of the conserved heme-binding motif in which H-P-G-G is replaced by H-P-A-G. Co-expression of the A. castellani Delta8-desaturase with the Isochrysis galbana Delta9-elongase in transgenic Arabidopsis plants confirmed the activity observed in yeast and its role in the alternative pathway for C20 polyunsaturated fatty acid synthesis. Acyl-CoA profiles of these transgenic plants revealed an unexpected accumulation of C20 fatty acids in the acyl-CoA pool. This is the first report of an alternative pathway C20 Delta8-desaturase from a non-photosynthetic organism, and also the first report of a front-end desaturase lacking the canonical cytochrome b5 domain.

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Citations

Nov 10, 2012·Applied Microbiology and Biotechnology·Pradinunt Eiamsa-ArdSireewan Kaewsuwan
May 3, 2007·Annual Review of Plant Biology·Johnathan A Napier
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Patricia VrintenXioa Qiu
Jun 23, 2007·Annals of the New York Academy of Sciences·John L Harwood
Nov 25, 2014·Applied Microbiology and Biotechnology·Noemi Ruiz-LopezRichard P Haslam
Dec 3, 2014·Journal of Agricultural and Food Chemistry·Helu LiuGuofan Zhang
Jan 16, 2007·The FEBS Journal·Verónica I LivoreAntonio D Uttaro
May 3, 2019·FEMS Microbiology Reviews·Ascel Samba-LouakaYann Héchard
Oct 17, 2012·Plant Biotechnology Journal·Richard P HaslamJohnathan A Napier
Aug 3, 2016·The Plant Journal : for Cell and Molecular Biology·Richard P HaslamJohnathan A Napier
May 21, 2011·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Olga Sayanova, Johnathan A Napier

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