The Ameliorating Effect of Sodium Selenite on the Histological Changes and Expression of Caspase-3 in the Testis of Monosodium Glutamate-Treated Rats: Light and Electron Microscopic Study

Journal of Microscopy and Ultrastructure
Nahla Reda Sarhan

Abstract

Monosodium glutamate (MSG) is a commonly used flavor enhancer that may contribute to male infertility. Sodium selenite is inorganic chemical form of selenium (Se). Se is best known as an antioxidant. This study was designed to investigate the possible ameliorating role of sodium selenite against MSG-induced testicular toxicity and histological changes. Forty male albino rats were allocated into four groups. Control group received distilled water, SE group received sodium selenite (0.25 mg/kg/day) dissolved in distilled water orally, MSG group received MSG (6 mg/g/day) dissolved in distilled water orally, and MSG + SE group received both MSG and sodium selenite for 45 days. Testicular samples were prepared for biochemical, light, and electron microscopic studies. Immunohistochemical staining for caspase-3 was done. MSG group demonstrated a significant increase in malondialdehyde level, marked damage of seminiferous tubules with a significant reduction in diameter and height of the lining epithelium. Spermatogenic cells showed disorganization, dark nuclei, reduction in number, and maturation arrest. Vacuolations of interstitial tissue and Leydig cells were also observed. Percent area of fibrosis and caspase-3 immunoexpression was...Continue Reading

Citations

Sep 6, 2020·Environmental Science and Pollution Research International·Fatma Abdel-Monem GadMahmoud Abdelghaffar Emam
Apr 16, 2021·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Javad SeyediJamshid Amiri Moghadam

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BETA
electron microscopic studies
MDA

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VideoTesT Morphology
Statistical Package for Social Science

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