The amelioration of renal damage in Skp2-deficient mice canceled by p27 Kip1 deficiency in Skp2-/- p27-/- mice.

PloS One
Sayuri SuzukiMasatoshi Kitagawa

Abstract

SCF-Skp2 E3 ubiquitin ligase (Skp2 hereafter) targets several cell cycle regulatory proteins for degradation via the ubiquitin-dependent pathway. However, the target-specific physiological functions of Skp2 have not been fully elucidated in kidney diseases. We previously reported an increase in Skp2 in progressive nephropathy and amelioration of unilateral ureteral obstruction (UUO) renal injury associated with renal accumulation of p27 in Skp2(-/-) mice. However, it remains unclear whether the amelioration of renal injury in Skp2(-/-) mice is solely caused by p27 accumulation, since Skp2 targets several other proteins. Using Skp2(-/-)p27(-/-) mice, we investigated whether Skp2 specifically targets p27 in the progressive nephropathy mediated by UUO. In contrast to the marked suppression of UUO renal injury in Skp2(-/-) mice, progression of tubular dilatation associated with tubular epithelial cell proliferation and tubulointerstitial fibrosis with increased expression of collagen and α-smooth muscle actin were observed in the obstructed kidneys in Skp2(-/-)p27(-/-) mice. No significant increases in other Skp2 target proteins including p57, p130, TOB1, cyclin A and cyclin D1 were noted in the UUO kidney in Skp2(-/-) mice, while ...Continue Reading

References

Aug 1, 1992·The Journal of Urology·G WaltonI S Sawczuk
Oct 1, 1995·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·E D Hay, A Zuk
Apr 1, 1995·Trends in Genetics : TIG·A Kamb
May 15, 1995·Genes & Development·C J Sherr, J M Roberts
Mar 9, 1995·Nature·D O Morgan
Apr 1, 1997·Immunology Today·A M Weissman
May 19, 1998·Nature Medicine·V OphascharoensukS J Shankland
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
Nov 13, 1999·Nature Cell Biology·A C CarranoM Pagano
Apr 3, 2001·Nature Cell Biology·J Bartek, J Lukas
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Saulo Klahr, Jeremiah Morrissey
May 6, 2003·The Journal of Biological Chemistry·Gil BornsteinAvram Hershko
Aug 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Takumi KamuraKeiichi I Nakayama
Dec 18, 2003·The Journal of Clinical Investigation·Raghu Kalluri, Eric G Neilson
Jan 31, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Michael Zeisberg, Raghu Kalluri
Sep 5, 2006·Cancer Research·Yoshihiro HiramatsuMasatoshi Kitagawa
Jul 11, 2007·The American Journal of Pathology·Sayuri SuzukiMasatoshi Kitagawa
May 27, 2008·Nature Reviews. Cancer·David Frescas, Michele Pagano
Aug 17, 2011·Cell and Tissue Research·Rosemarie M CarewPhillip Kantharidis
Oct 25, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Sayuri SuzukiMasatoshi Kitagawa
Dec 14, 2011·Trends in Cell Biology·Natalia G Starostina, Edward T Kipreos

❮ Previous
Next ❯

Citations

Dec 21, 2012·Cellular and Molecular Life Sciences : CMLS·Sayuri SuzukiMasatoshi Kitagawa
Feb 11, 2016·The International Journal of Biochemistry & Cell Biology·Nana ZhengWenyi Wei
Mar 25, 2014·Nature Reviews. Cancer·Zhiwei WangWenyi Wei
Feb 13, 2020·Scientific Reports·Sayuri SuzukiAndrea Fleig
Jul 3, 2021·International Journal of Molecular Sciences·Naoki InuiMasatoshi Kitagawa

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
protein assay

Software Mentioned

SAS
Scion Image
StatView

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2021 Meta ULC. All rights reserved