Abstract
Whole cell voltage clamp recordings were performed to assess the ability of conantokin-G (con-G), conantokin-T (con-T), and a 17-residue truncated form of conantokin-R (con-R[1-17]) to inhibit N-methyl-d-aspartate (NMDA)-evoked currents in human embryonic kidney 293 cells transiently expressing various combinations of NR1a, NR1b, NR2A, and NR2B receptor subunits. Con-T and con-R[1-17] attenuated ion currents in cells expressing NR1a/NR2A or NR1a/NR2B. Con-G did not affect NMDA-evoked ionic currents in cells expressing NR1a/NR2A, but it showed inhibitory activity in cells expressing NR1a/NR2B receptors and the triheteromeric combination of NR1a/NR2A/NR2B. An Ala-rich con-G analog, con-G[Q6G/gamma7K/N8A/gamma10A/gamma14A/K15A/S16A/N17A] (Ala/con-G, where gamma is Gla), in which all nonessential amino acids were altered to Ala residues, manifested subunit specificity similar to that of con-G, suggesting that the replaced residues are not responsible for selectivity in the con-G framework. A sarcosine-containing con-T truncation analog, con-T[1-9/G1Src/Q6G], inhibited currents in NR1a/NR2A and NR1a/NR2B receptors, eliminating residues 10-21 as mediators of the broad subunit selectivity of con-T. In contrast to the null effects of c...Continue Reading
References
Oct 1, 1992·Journal of Neurochemistry·P SkolnickM L Maccecchini
May 22, 1992·Science·H MonyerP H Seeburg
Jun 30, 1992·Biochemical and Biophysical Research Communications·H SugiharaS Nakanishi
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G M DurandR S Zukin
Feb 5, 1987·Nature·C E Jahr, C F Stevens
Feb 5, 1987·Nature·J W Johnson, P Ascher
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Aug 16, 1994·European Journal of Pharmacology·D J Laurie, P H Seeburg
Apr 1, 1995·Journal of Neurochemistry·D R LynchD B Pritchett
Sep 30, 1993·Neuroreport·K A WaffordJ A Kemp
Jun 1, 1993·International Journal of Peptide and Protein Research·T L Colpitts, F J Castellino
Aug 1, 1996·Journal of Neurochemistry·S J KendrickD B Pritchett
Sep 1, 1996·International Journal of Peptide and Protein Research·M ProrokF J Castellino
Dec 24, 1996·Biochemistry·M ProrokF J Castellino
Jan 1, 1997·Molecular Pharmacology·J LuoB B Wolfe
Mar 1, 1997·Neuron·B LaubeJ Kuhse
Jun 1, 1997·Molecular Pharmacology·N ChenL A Raymond
Jan 14, 1998·Brain Research. Molecular Brain Research·D J LaurieP H Seeburg
Apr 29, 1998·The Journal of Biological Chemistry·S E WarderF J Castellino
Jul 25, 1998·The Journal of Biological Chemistry·M Prorok, F J Castellino
Oct 8, 1998·FEBS Letters·T BlandlF J Castellino
Jan 19, 1999·FEBS Letters·L SunM S Lidow
Sep 10, 1999·The Journal of Biological Chemistry·L M HawkinsF A Stephenson
Oct 9, 1999·Neuroscience Letters·R C Klein, F J Castellino
Dec 23, 1999·Neuropharmacology·R C KleinF J Castellino
Mar 29, 2000·FEBS Letters·T BlandlF J Castellino
Aug 23, 2000·Molecular Pharmacology·S D Donevan, R T McCabe
Sep 21, 2000·European Journal of Pharmacology·A C AdamsK A Keefe
Nov 30, 2000·The Journal of Biological Chemistry·T BlandlF J Castellino
May 2, 2001·Journal of Neurochemistry·S E WarderM Prorok
Citations
Jul 25, 2006·Cell and Tissue Research·Georg Köhr
May 19, 2012·Biochemistry·Konkallu Hanumae GowdGrzegorz Bulaj
Aug 19, 2010·Pharmacological Reviews·Stephen F TraynelisRay Dingledine
Nov 14, 2013·Amino Acids·Michael J EspirituJon-Paul Bingham
Mar 25, 2009·Biochemistry·Vernon D TwedeBaldomero M Olivera
Jun 9, 2004·European Journal of Biochemistry·Emma L MillardDavid J Craik
May 20, 2003·The Journal of Peptide Research : Official Journal of the American Peptide Society·R C KleinF J Castellino
Jul 1, 2004·Toxicon : Official Journal of the International Society on Toxinology·Wudayagiri RajendraKandiah Jeyaseelan
Jan 1, 2003·Pain·Annika B MalmbergAllan I Basbaum
Sep 6, 2006·Toxicon : Official Journal of the International Society on Toxinology·Raymond S Norton, Baldomero M Olivera
Jul 25, 2006·Neuroscience Letters·Juanjuan WeiQiuyun Dai
Jan 6, 2005·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Elisa A Waxman, David R Lynch
Jul 9, 2004·Journal of Neurophysiology·Lijun LiLynn A Raymond
Jul 22, 2014·Journal of Neuroscience Research·Luoxiu HuangFrancis J Castellino
Aug 11, 2006·Journal of Neurochemistry·Yi Na DongDavid R Lynch
Jun 22, 2005·The Journal of Biological Chemistry·Elisa A Waxman, David R Lynch
Dec 13, 2005·Journal of Neurochemistry·L RagnarssonD J Adams
Jun 9, 2006·Journal of Neurophysiology·Anitha B AlexKaren S Wilcox
Aug 4, 2006·Journal of Neurophysiology·Congyi LuStefano Vicini
Mar 13, 2012·Pharmacological Reviews·Richard J LewisMacDonald J Christie
Sep 30, 2017·Frontiers in Cellular Neuroscience·Lei-Lei LiuDao-Qi Zhang
Jan 13, 2004·Physiological Reviews·Heinrich Terlau, Baldomero M Olivera
May 18, 2017·BMC Neuroscience·Edwin A Reyes-GuzmanEsperanza Recio-Pinto
Mar 10, 2007·The Journal of Biological Chemistry·Qiuyun DaiMary Prorok
Jun 9, 2020·ACS Nano·Pierluigi ValenteRoberto Fiammengo
Jan 21, 2014·Neuropharmacology·Rashna D BalsaraPatrick L Sheets
Jan 18, 2006·Neuroscience·A J IrvingJ Harvey
Oct 14, 2010·ACS Nano·Lisa MausRoberto Fiammengo
Jun 26, 2007·Neuropharmacology·Zhenyu ShengFrancis J Castellino
Jul 8, 2008·Neuropharmacology·Zhenyu ShengFrancis J Castellino
May 12, 2009·Peptides·Qiuyun DaiMary Prorok
May 12, 2009·Neuropharmacology·Zhenyu ShengFrancis J Castellino
Jul 6, 2010·Molecular and Cellular Neurosciences·Luoxiu HuangFrancis J Castellino
Aug 7, 2010·Neuroscience·Z ShengF J Castellino