The amino-acid sequence of isoinhibitor K form snails (Helix pomatia). A sequence determination by automated Edman degradation and mass-spectral identification of the phenylthiohydantoins.

European Journal of Biochemistry
H Tschesche, T Dietl

Abstract

Performic-acid-oxidized isoinhibitor K of snails (Helix pomatia) was subjected to arginine-directed tryptic proteolysis. Six peptide fragments including one overlap peptide from limited cleavage of the Arg-3-Pro-4 bond were purified to homogeneity. Four arginine peptides and the C-terminal peptide were sequenced by automatic Edman degradation using a special peptide program. The phenylthiohydantoins were all identified by chemical ionization mass spectrometry, except four cysteic acid residues that were identified on an amino acid analyzer after acid hydrolysis. Quantitative evaluation of the phenylthiohydantoins by chemical ionization mass spectrometry using total molecular-ion beam integration greatly facilitated sequencing. The mass spectrum of the dipeptide less than Glu-Gly revealed that the N-terminus was blocked by pyroglutamic acid. The complete amino acid sequence of isoinhibitor K was determined. An almost 50% homology between the sequences of the snail inhibitor and the bovine trypsin-kallikrein inhibitor (Kunitz) became obvious. A comparison of all homologous sequences of this particular class of proteins known to date is presented.

References

Jan 1, 1968·Biochemistry·A L Grossberg, D Pressman
Mar 15, 1972·European Journal of Biochemistry·H Tschesche, S Kupfer
Mar 1, 1968·European Journal of Biochemistry·B MelounF Sorm
Sep 1, 1968·The Biochemical Journal·H B Dixon, R N Perham
Aug 16, 1965·Biochemical and Biophysical Research Communications·B Kassell, M Laskowski
Mar 1, 1967·European Journal of Biochemistry·P Edman, G Begg

❮ Previous
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Citations

Jan 1, 1980·The International Journal of Biochemistry·D M Kirschenbaum
Oct 1, 1995·Molecular and Biochemical Parasitology·S E BozasT W Spithill
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Y GonzálezM A Chávez
Jan 23, 1999·European Journal of Biochemistry·A KuriokaH Hirano
Mar 7, 2001·European Journal of Biochemistry·G T NagleK W Li
Mar 1, 1995·Genetic Analysis : Biomolecular Engineering·N G HommesL A Sayavedra-Soto
Nov 1, 1990·Journal of Biochemical and Biophysical Methods·B Persson, D Eaker
Jan 1, 1985·The International Journal of Biochemistry·D X ZhuK K Han
Nov 14, 1978·Biochemical and Biophysical Research Communications·P Hallenbeck
Feb 26, 2015·PLoS Computational Biology·Jagannath SwaminathanEdward M Marcotte
Jan 18, 2006·Biochemical and Biophysical Research Communications·Sergio D SasakiAparecida S Tanaka
Jan 5, 2002·Biological & Pharmaceutical Bulletin·A SandaM Irie
Mar 5, 1987·Journal of Molecular Biology·T E Creighton, I G Charles

❮ Previous
Next ❯

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