The amino-acid sequence of rabbit Cu-Zn superoxide dismutase

Biological Chemistry Hoppe-Seyler
K ReineckeL Flohé

Abstract

The primary structure of Cu-Zn superoxide dismutase from rabbit liver was investigated. The reduced and S-carboxymethylated enzyme was treated with cyanogen bromide, trypsin or Staphylococcus aureus proteinase V8. The resulting peptides were separated by high-performance liquid chromatography and sequenced by automated Edman degradation. With the exception of the N- and C-terminus the complete sequence was established by means of overlapping peptides. The N-terminus is blocked and thus not susceptible to Edman degradation. The amino-acid composition of the tryptic N-terminal peptide corresponds to that of the cytoplasmatic Cu-Zn superoxide dismutases of other mammals investigated. The chromatographic behaviour of these N-terminal peptides on a reversed phase C18 column is also identical, thus suggesting also for the rabbit Cu-Zn superoxide dismutase the N-terminal sequence Ac-Ala-Thr-Lys. The C-terminus was demonstrated to have the sequence -Ile-Ala-Pro by enzymatic degradation with carboxypeptidase Y. The complete amino-acid sequence of the rabbit Cu-Zn superoxide dismutase consists of 152 amino-acids and shows the expected homology to other Cu-Zn enzymes published so far. The aspartate and six histidine residues known to comp...Continue Reading

References

Nov 1, 1979·Hoppe-Seyler's Zeitschrift für physiologische Chemie·G C SteffensG Buse
Oct 1, 1986·Biological Chemistry Hoppe-Seyler·G J SteffensL Flohé
Sep 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·G J SteffensL Flohé

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Citations

Aug 1, 1993·Biological Chemistry Hoppe-Seyler·B WolfL Flohé
Jan 1, 1991·Free Radical Research Communications·J KwiatowskiF J Ayala
Nov 24, 1999·Virology·D O WillerD H Evans
Oct 1, 1991·The European Journal of Neuroscience·Tommy AnderssonKrister Kristensson
Nov 1, 1995·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P CalabresiG Bernardi
Sep 20, 1996·Journal of Chromatography. B, Biomedical Applications·W P Michalski

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