The amino acid sequence of the alpha chain of the major haemoglobin of the rat (Rattus norvegicus).

The Biochemical Journal
C G ChuaB H Howard

Abstract

1. A partial amino acid sequence of the alpha chain from the rat (Wistar, Rattus norvegicus) major haemoglobin is reported. The soluble tryptic peptides prepared from aminoethylated alpha-globin were separated by peptide 'mapping'. Sequencing of the tryptic peptides was carried out by the dansyl-Edman method and by the overlapping of smaller peptide fragments derived from secondary enzymic digestion. The insoluble 'core' peptides were further digested with chymotrypsin, thermolysin and pepsin to give smaller soluble peptides for sequencing. The tryptic peptides were ordered on the basis of their homology with the corresponding peptides of human alpha chain. 2. The proposed sequence is compared with that obtained by using an automated sequencer [Garrick et al. (1975) Biochem. J. 149, 245-258]. The differences in sequence resulting from the two methods are discussed. 3. It is suggested that the externally situated cysteine (residue 13) is responsible for the observed inhibition of crystallization of rat haemoglobin at alkaline pH. 4. Detailed evidence for the sequence has been deposited as Supplementary Publication SUP 50047 (9 pages) at the British Library (Linding Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., ...Continue Reading

Citations

Aug 1, 1977·European Journal of Biochemistry·N S KosowerR L Koppel
Jan 1, 1984·Hemoglobin·R CrkvenjakovV Glisin
May 17, 1982·European Journal of Biochemistry·M E John
Dec 15, 1982·European Journal of Biochemistry·M BrunoriB Giardina
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Aug 31, 1978·Nature·S O Brennan, R W Carrell
Sep 12, 2015·The Journal of Toxicological Sciences·Yasuhiro ShinkaiYoshito Kumagai
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Aug 1, 1985·The American Journal of Physiology·S A HendersonG A Brooks
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Jul 31, 1987·Biochemical and Biophysical Research Communications·H SatohT Okazaki
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