PMID: 9172081Jun 1, 1997Paper

The amino acid sequence of the light chain of Acanthamoeba myosin IC

Journal of Muscle Research and Cell Motility
Z Y WangE D Korn

Abstract

The amino acid sequence of the light chain of Acanthamoeba myosin IC deduced from the cDNA sequence comprises 149 amino acids with a calculated molecular weight of 16,739. All but the 3 N-terminal residues were also determined by amino acid sequencing of the purified protein, which also showed the N-terminus to be blocked. Phylogenetic analysis shows Acanthamoeba myosin IC light chain to be more similar to the calmodulin subfamily of EF-hand calcium-modulated proteins than to the myosin II essential light chain or regulatory light chain subfamilies. In pairwise comparisons, the myosin IC light chain sequence is most similar to sequences of calmodulins (approximately 50% identical) and a squid calcium-binding protein (approximately 43% identical); the sequence is approximately 37% identical to the calcium-binding essential light chain of Physarum myosin II and approximately 30% identical to the essential light chain of Acanthamoeba myosin II, and the essential light chain and regulatory light chain of Dictyostelium myosin II. The sequence predicts four helix-loop-helix domains with possible calcium-binding sites in domains I and III, suggesting that calcium may affect the activity of this unconventional myosin. This is the first...Continue Reading

Citations

Mar 31, 1998·Cell Motility and the Cytoskeleton·K M MayJ S Hyams
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z Y WangJ A Hammer
Mar 26, 2002·FEBS Letters·Martin Bähler, Allen Rhoads
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·B O CarragherA C Steven
Jul 15, 2015·Bioarchitecture·Sarah M Heissler, James R Sellers
Aug 12, 1998·The Journal of Cell Biology·R C Stevens, T N Davis
Mar 30, 2001·The Journal of Biological Chemistry·M S Rogers, E E Strehler
Aug 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takashi IshikawaAlasdair C Steven
Nov 13, 2007·Journal of Muscle Research and Cell Motility·Nanyun TangE Michael Ostap
Jul 14, 1999·Cell Biochemistry and Biophysics·T SoldatiE C Schwarz
Mar 22, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M ToyaM Yamamoto
Oct 29, 2005·The Journal of Biological Chemistry·Tianming LinE Michael Ostap
Jan 12, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kae-Jung HwangJames M Gruschus
Jun 23, 2011·Journal of Cell Science·Matthew R SammonsMatthew Lord
Mar 21, 2001·Biochimica Et Biophysica Acta·M A de la Roche, G P Côté
Mar 21, 2000·Biochimica Et Biophysica Acta·B BarylkoJ P Albanesi

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