PMID: 9163340Apr 15, 1997Paper

The amino-terminal module of the C4b-binding protein alpha-chain is crucial for C4b binding and factor I-cofactor function

The Biochemical Journal
Y HärdigB Dahlback

Abstract

C4b-binding protein (C4BP) regulates the classical pathway C3-convertase of the complement system. Human C4BP is composed of seven identical subunits (alpha-chains) and one unique one (beta-chain). Both types of chains contain homologous repeats called complement control proteins (CCPs); the alpha-chain contains eight CCPs and the beta-chain three. Each alpha-chain contains a binding site for C4b although the detailed localization of this binding site is not known. We have used three different chimeric proteins, originally designed to localize the protein S-binding site on C4BP, to demonstrate the importance of the amino-terminal part of the alpha-chain for the complement-regulatory functions of C4BP. These recombinant proteins were composed of C4BP alpha-chains with one, two or three of the amino-terminal CCPs replaced by corresponding CCPs from the C4BP beta-chain. Furthermore, seven different monoclonal antibodies were raised against C4BP and characterized using the recombinant chimeric proteins. Whereas all three recombinant chimeras bind protein S with the same affinity as plasma-purified C4BP, none of them bound to C4b. Three of the antibodies, which were found to bind to alpha-chain CCP 1 and CCP 2, completely inhibited ...Continue Reading

Citations

May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A M BlomA B Jonsson
May 23, 2001·The Journal of Biological Chemistry·A M BlomB Dahlbäck
May 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Srinjoy ChakrabortiSanjay Ram
Jun 25, 2005·Protein Engineering, Design & Selection : PEDS·Dinesh C SoaresPaul N Barlow
Sep 18, 2015·Microbiology·Sara Schesser BartraGregory V Plano
Apr 8, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·David ErmertAnna M Blom
Sep 30, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maisem LaabeiAnna M Blom
Sep 29, 2020·Frontiers in Immunology·Maisem LaabeiAnna M Blom
Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joanna H WebbBjörn Dahlbäck
Aug 30, 2003·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Joanna H WebbBjörn Dahlbäck
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nemani V PrasadaraoLinette C Linsangan
Jun 7, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Panisadee AvirutnanJohn P Atkinson
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A M BlomB Dahlbäck
Jul 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M T HolmbergS Meri
May 10, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanna JarvaSeppo Meri
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanna JarvaAnna M Blom
Nov 1, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Somchai ThiemmecaPanisadee Avirutnan
May 23, 2001·International Immunopharmacology·S RamP A Rice
Dec 15, 2015·Immunology Letters·David Ermert, Anna M Blom

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