The aminoglycoside antibiotic gentamicin is able to alter metabolic activity and morphology of MDCK-C11 cells: a cell model of intercalated cells

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
A G G CoutinhoA L Tararthuch

Abstract

It is well known that the aminoglycoside antibiotic gentamicin is capable of causing damage to kidney cells. Given the known involvement of Ca2+ in the nephrotoxic action of gentamicin, the purpose of this study was to establish a relationship between the concentration of intracellular Ca2+ ([Ca2+]i) and cellular cytotoxicity using MDCK-C11 cells, a clone that has several properties that resemble those of intercalated cells of the distal nephron. Changes in [Ca2+]i was determined using fluorescence microscopy. Cell viability was evaluated by the neutral red method, and cell cytotoxicity by the MTT method. The [Ca2+]i gradually increased when cells were exposed to 0.1 mM gentamicin for 10, 20, and 30 min. The presence of extracellular Ca2+ was found to be necessary to stimulate the increase in [Ca2+]i induced by gentamicin, since this stimulus disappeared by using 1.8 mM EGTA (a Ca2+ chelator). Morphological changes were observed with scanning electron microscopy in epithelial cells exposed to the antibiotic. Furthermore, with the MTT method, a decrease in metabolic activity induced by gentamicin was observed, which indicates a cytotoxic effect. In conclusion, gentamicin was able to alter [Ca2+]i, change the morphology of MDCK-C...Continue Reading

References

Sep 1, 1995·The Journal of Clinical Investigation·S K MoestrupE I Christensen
Sep 1, 1994·Pflügers Archiv : European journal of physiology·M GekleS Silbernagl
May 10, 2000·The Journal of Biological Chemistry·A Woods, J R Couchman
May 10, 2000·The Journal of Biological Chemistry·E F PlowJ W Smith
Feb 24, 2001·Pflügers Archiv : European journal of physiology·R FernandezG Malnic
Apr 9, 2001·American Journal of Physiology. Renal Physiology·K A BlankenshipJ M Arthur
Apr 1, 2005·Journal of the American Society of Nephrology : JASN·Donald T WardDaniela Riccardi
Jun 1, 2005·Hearing Research·Sigrid E Myrdal, Peter S Steyger
Oct 4, 2005·The Journal of Clinical Investigation·Chunyan XuJohn C Reed
Aug 7, 2008·Journal of Cell Science·Takatoshi KarasawaPeter S Steyger
Jan 21, 2009·The EMBO Journal·Tomasz WegierskiMichael Köttgen
May 4, 2010·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Pitchai BalakumarArunachalam Thangathirupathi
Sep 11, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yaremi QuirosFrancisco J López-Hernández

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Citations

Nov 1, 2020·Environmental Science and Pollution Research International·Emad H M HassaneinOmnia A M Abd El-Ghafar

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Methods Mentioned

BETA
scanning electron microscopy

Software Mentioned

AxioVision
GraphPad InStat
GraphPad

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