The Amotl2 gene inhibits Wnt/β-catenin signaling and regulates embryonic development in zebrafish.

The Journal of Biological Chemistry
Zhiqiang LiAnming Meng

Abstract

The Motin family proteins can regulate cell polarity, cell mobility, and proliferation during embryonic development by controlling distinct signaling pathways. In this study, we demonstrate that amotl2 knockdown in zebrafish wild-type embryos results in embryonic dorsalization, and this effect can be antagonized by co-knockdown of the dorsal inducer β-catenin2. Overexpression of amotl2 in masterblind (mbl) homozygous embryos, in which canonical Wnt signaling is up-regulated due to an axin1 mutation, transforms eyeless phenotype into smaller eyes, whereas co-knockdown of amot, amotl1, and amotl2 leads to development of smaller eyes in mbl heterozygotes. In cultured mammalian cells, Motin family members all possess the ability to attenuate Wnt/β-catenin signaling. Focusing on Amotl2, we show that Amotl2 can associate with and trap β-catenin in the Rab11-positive recycling endosomes, and as a result, the amount of β-catenin in the cytosol and nucleus is reduced. Thus, our findings provide novel insights into functions of Motin family members and regulation of Wnt/β-catenin signaling.

References

Sep 5, 2001·Current Opinion in Genetics & Development·J Huelsken, W Birchmeier
Oct 28, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tinchung LeungWolfgang Driever
Feb 13, 2004·Developmental Cell·Stephen W Wilson, Corinne Houart
Jul 23, 2004·Development·Marie-Christine Ramel, Arne C Lekven
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Feb 19, 2005·Science·D D SarbassovDavid M Sabatini
Jul 27, 2005·The Journal of Biological Chemistry·Anders BrattLars Holmgren
Dec 3, 2005·The International Journal of Developmental Biology·Claudio D SternLewis Wolpert
Dec 27, 2005·Current Opinion in Genetics & Development·Felix H BrembeckWalter Birchmeier
Apr 28, 2006·The FEBS Journal·Mira ErnkvistLars Holmgren
Jan 2, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ying SuYe-Guang Chen
Aug 19, 2007·Genes & Development·Karin AaseLars Holmgren
Oct 22, 2008·The Journal of Biological Chemistry·Xia GaoYe-Guang Chen
Nov 26, 2008·Cold Spring Harbor Symposia on Quantitative Biology·R NusseY Kalani
Jun 11, 2009·Journal of Cell Science·Yu ZhangAnming Meng
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Jan 19, 2010·The Journal of Biological Chemistry·Brigitte HellerClark D Wells
Apr 24, 2010·Developmental Cell·Xaralabos VarelasLiliana Attisano
Dec 29, 2010·The Journal of Biological Chemistry·Wenqi WangJunjie Chen
Jan 6, 2011·Genes & Development·Bin ZhaoKun-Liang Guan
Jan 13, 2011·The Journal of Biological Chemistry·Siew Wee ChanWanjin Hong
Mar 23, 2011·The Journal of Cell Biology·Buzz Baum, Marios Georgiou
May 13, 2011·Current Drug Targets·Oscar Pellón-CárdenasCrislyn D'Souza-Schorey
Aug 13, 2011·Molecular Biology of the Cell·Murugan ParamasivamDannel McCollum
Sep 29, 2011·Annual Review of Genetics·Yvette G Langdon, Mary C Mullins

❮ Previous
Next ❯

Citations

Oct 19, 2012·PloS One·Galen W MillerMaret G Traber
Aug 13, 2013·PloS One·Emily H M WongMaria-Mercè Garcia-Barceló
Oct 13, 2017·Molecular Biology of the Cell·Nicolas AznarPradipta Ghosh
Feb 20, 2014·FEBS Letters·Susana MoleirinhoJoseph L Kissil
Aug 16, 2018·PloS One·Anastasia M SorokinaJustin S Rhodes
Oct 31, 2018·Genesis : the Journal of Genetics and Development·Ryan P LiegelRolf W Stottmann
Jun 4, 2019·Reproduction in Domestic Animals = Zuchthygiene·Pengfei ShiFenghua Lu
Apr 14, 2018·Journal of Translational Medicine·Tingting HuangWei Kang
Aug 24, 2018·Nature Communications·Marissa A ScavuzzoMalgorzata Borowiak
Oct 8, 2020·American Journal of Medical Genetics. Part a·Jonathan RipsTamar Harel
Feb 27, 2021·Frontiers in Cell and Developmental Biology·Brian S ClarkBrian A Link
Mar 26, 2013·Journal of Cell Science·Tomasz J Proszynski, Joshua R Sanes
May 18, 2021·Frontiers in Cell and Developmental Biology·Wojciech J SzlachcicMalgorzata Borowiak
Jun 1, 2021·The Journal of Biological Chemistry·Sayantani GoswamiNan Gao
Aug 16, 2021·Neurobiology of Learning and Memory·Vern LewisAndreas Heyland

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.