The AMPK β2 subunit is required for energy homeostasis during metabolic stress.

Molecular and Cellular Biology
Biplab DasguptaJeffrey Milbrandt

Abstract

AMP activated protein kinase (AMPK) plays a key role in the regulatory network responsible for maintaining systemic energy homeostasis during exercise or nutrient deprivation. To understand the function of the regulatory β2 subunit of AMPK in systemic energy metabolism, we characterized β2 subunit-deficient mice. Using these mutant mice, we demonstrated that the β2 subunit plays an important role in regulating glucose, glycogen, and lipid metabolism during metabolic stress. The β2 mutant animals failed to maintain euglycemia and muscle ATP levels during fasting. In addition, β2-deficient animals showed classic symptoms of metabolic syndrome, including hyperglycemia, glucose intolerance, and insulin resistance when maintained on a high-fat diet (HFD), and were unable to maintain muscle ATP levels during exercise. Cell surface-associated glucose transporter levels were reduced in skeletal muscle from β2 mutant animals on an HFD. In addition, they displayed poor exercise performance and impaired muscle glycogen metabolism. These mutant mice had decreased activation of AMPK and deficits in PGC1α-mediated transcription in skeletal muscle. Our results highlight specific roles of AMPK complexes containing the β2 subunit and suggest th...Continue Reading

References

Oct 1, 1992·The Journal of Clinical Investigation·V SarabiaA Klip
Sep 1, 1997·Metabolism: Clinical and Experimental·S M FurlerE W Kraegen
Dec 18, 1998·The Journal of Biological Chemistry·B E CruteL A Witters
Sep 16, 2000·The EMBO Journal·M C Schmidt, R R McCartney
Nov 18, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J Y KimJ O Holloszy
Jan 4, 2003·The Journal of Clinical Investigation·Benoit ViolletSophie Vaulont
Mar 29, 2003·Diabetes·Sean L McGeeMark Hargreaves
May 16, 2003·Current Biology : CB·Galina PolekhinaDavid Stapleton
Mar 26, 2004·Reviews in Endocrine & Metabolic Disorders·D Grahame Hardie
Jul 22, 2004·American Journal of Physiology. Endocrinology and Metabolism·Ji LiLawrence H Young
Apr 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brian R BarnesJuleen R Zierath
May 10, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sebastian B JørgensenHenriette Pilegaard
Oct 27, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pieter de LangeFernando Goglia
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Biplab Dasgupta, Jeffrey Milbrandt
May 5, 2007·Diabetes Research and Clinical Practice·Jongsoon Lee, Myung-Sunny Kim
Jul 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sibylle JägerBruce M Spiegelman
Aug 23, 2007·The Journal of Biological Chemistry·Eric L GreerAnne Brunet
Feb 8, 2008·American Journal of Physiology. Renal Physiology·Philippe G CammisottoMoïse Bendayan
Apr 29, 2008·Molecular Cell·Dana M GwinnReuben J Shaw
Jun 20, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Milos R SpasićKoenraad K Norga
Aug 5, 2008·Cell·Vihang A NarkarRonald M Evans
Oct 31, 2008·Endocrine Reviews·Marc-Andre CornierRobert H Eckel
Aug 28, 2009·Cell Biochemistry and Function·Eduardo KokubunHorst Haebisch
Nov 20, 2009·American Journal of Physiology. Cell Physiology·Jonas T TreebakLaurie J Goodyear
Sep 22, 2010·The Journal of Biological Chemistry·Gregory R SteinbergBruce E Kemp
Nov 3, 2010·Cell Metabolism·Michale BouskilaKei Sakamoto
Nov 17, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·R S Lee-YoungD H Wasserman

❮ Previous
Next ❯

Citations

Jul 19, 2013·The Journal of Clinical Investigation·Neil B RudermanJosé M Cacicedo
Jul 25, 2013·International Journal of Molecular Sciences·Pieter de LangeAntonia Lanni
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xiaona LiuBiplab Dasgupta
Sep 10, 2014·Nature Neuroscience·Bogdan BeirowskiJeffrey Milbrandt
Sep 1, 2015·Adipocyte·Xin YangShihuan Kuang
Feb 27, 2013·Diabetes & Metabolism Journal·Hayley M O'Neill
Jan 5, 2013·Journal of Applied Physiology·David Morales-AlamoJosé A L Calbet
May 24, 2014·Journal of Applied Physiology·Adam L BujakGregory R Steinberg
Oct 21, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Richard T JaspersPieter de Lange
Jan 5, 2020·Molecular & Cellular Proteomics : MCP·Zhen ChenJunjie Chen
Aug 19, 2020·Journal of Hematology & Oncology·Jimin YuanJiancheng Hu
Jan 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Richard T JaspersPieter de Lange
Aug 11, 2020·Cancer Cell International·Xixia ZhangZhiwei Cao
Sep 10, 2020·Frontiers in Cell and Developmental Biology·Abitha SukumaranBiplab Dasgupta
Oct 20, 2018·Frontiers in Physiology·Bahareldin A AbdallaXiquan Zhang
Oct 31, 2018·International Journal of Molecular Sciences·Natalie R JanzenNolan J Hoffman
Jun 1, 2013·The FEBS Journal·Pascual SanzMaria Adelaida Garcia-Gimeno
Jul 8, 2020·International Journal of Molecular Sciences·Boris Y ShorningHelen B Pearson
Sep 3, 2020·Cell Reports·Ranjithmenon MuraleedharanBiplab Dasgupta
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Zhen ChenJunjie Chen
Oct 9, 2019·Aging and Disease·Brian Z WangKunlin Jin
Jul 30, 2021·Arthritis Research & Therapy·M BadiiL A B Joosten

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.