The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box

Life
Paulo OliveiraPaula Tamagnini

Abstract

The interest in examining the subset of proteins present in the extracellular milieu, the exoproteome, has been growing due to novel insights highlighting their role on extracellular matrix organization and biofilm formation, but also on homeostasis and development. The cyanobacterial exoproteome is poorly studied, and the role of cyanobacterial exoproteins on cell wall biogenesis, morphology and even physiology is largely unknown. Here, we present a comprehensive examination of the Anabaena sp. PCC 7120 exoproteome under various growth conditions. Altogether, 139 proteins belonging to 16 different functional categories have been identified. A large fraction (48%) of the identified proteins is classified as "hypothetical", falls into the "other categories" set or presents no similarity to other proteins. The evidence presented here shows that Anabaena sp. PCC 7120 is capable of outer membrane vesicle formation and that these vesicles are likely to contribute to the exoproteome profile. Furthermore, the activity of selected exoproteins associated with oxidative stress has been assessed, suggesting their involvement in redox homeostasis mechanisms in the extracellular space. Finally, we discuss our results in light of other cyano...Continue Reading

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Citations

Jul 5, 2016·Frontiers in Microbiology·Joaquín Giner-LamiaPaulo Oliveira
Mar 11, 2017·Molecular Phylogenetics and Evolution·Ângela BritoPaula Tamagnini
Oct 27, 2015·The ISME Journal·Rhona K StuartMichael P Thelen
Mar 9, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Neelam DubeySuvendra Nath Bagchi
Dec 13, 2019·Carbohydrate Polymers·Rita MotaPaula Tamagnini
Apr 22, 2021·Journal of Microbiology and Biotechnology·Hannah SchätzleEnrico Schleiff

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Methods Mentioned

BETA
electrophoresis
transmission
mechanical shearing

Software Mentioned

EasyProt
PSORTb
[UNK]

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