The analysis of an anti-fluorescein cytotoxic response

European Journal of Immunology
N ChristensenJ Marbrook

Abstract

The detailed kinetics of the appearance of clones of anti-fluorescein cytotoxic T lymphocytes (CTL) in a primary limiting dilution response have been examined. The addition of factors from the supernatants of concanavalin A-stimulated rat spleen cell cultures was necessary to obtain linear dose-response curves and strong primary responses. In a standard primary response the maximum detectable number of specific clones was found, on day 5, to be derived from precursors having a frequency of 1 in 10 000 spleen cells. When limiting dilution cultures were restimulated on day 3, responses on day 5 were unaffected but new clones appeared on day 7 with a frequency of 1 in 1000. The emergence of the day-7 response depends on the addition of factor(s) on day 3. It is proposed that the early low frequency precursors detected on day 5 and the high frequency precursors detected on days 7-9 are derived from two stages in the differentiation lineage of CTL precursors. Restimulation of limiting dilution cultures involves not only the continued clonal expansion of primary responses but also the emergence of new "clones" of specific CTL.

References

Jan 1, 1979·European Journal of Immunology·L M Ching, J Marbrook
Jun 1, 1976·The Journal of Experimental Medicine·M A Skinner, J Marbrook
Aug 1, 1974·European Journal of Immunology·G M Shearer
Jun 1, 1980·The Journal of Experimental Medicine·L A Sherman
Jan 1, 1980·Immunological Reviews·H R MacDonaldK T Brunner
Aug 1, 1980·European Journal of Immunology·G Dennert, R Hyman
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Aug 13, 1982·Journal of Immunological Methods·W F ChenK Shortman
Mar 12, 1982·Journal of Immunological Methods· Fazekas de St Groth

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Citations

Nov 1, 1992·Veterinary Immunology and Immunopathology·D M Dunham, E C Hahn
Jan 1, 1989·International Journal of Immunopharmacology·M SkinnerJ Marbrook
Jan 15, 1986·International Journal of Cancer. Journal International Du Cancer·M Zöller
Apr 1, 1985·European Journal of Immunology·J ReimannH Wagner

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