The analysis of biofilms Vibrio choleraе using technique of transmissive electronic microscopy

Klinicheskaia laboratornaia diagnostika
S N GolovinA V Trishina

Abstract

The cholera continues to be one of the priority problems of world health care that determines necessity of its permanent monitoring and also further investigation of this infection in microbiological and ecological aspects. The phenomenon of biofilm formation plays a significant role among mechanisms permitting comma bacillus year by year to retain its ecological niche and to spread in various basins over a period of decades. The application of techniques of electronic microscopy played a decisive role in studying the process of biofilm formation. The transmission electronic microscopy has a number of significant advantages, but studying of biofilms using the given technique has certain limitations and peculiarities affecting derivable results. The purpose of study is modeling of biofilms of comma bacillus using a new original technique and analyzing them implementing transmission electronic microscopy. An original substrate was developed to cultivate biofilms of comma bacillus adjusted to convenient sampling preparation for transmission electronic microscopy. The application of the given technique manifold simplifies the process sampling preparation and permits to retain at most the native structure of biofilms. By means of co...Continue Reading

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