The analysis of virulence factors and antibiotic resistance between Helicobacter pylori strains isolated from gastric antrum and body

BMC Gastroenterology
Ji Won SeoJae Gyu Kim

Abstract

Individuals can be infected with multiple strains of Helicobacter pylori. However, the differences among co-infecting strains have not been well analyzed yet. This study aimed to investigate whether the virulence factors and antibiotic resistance patterns of H. pylori differ between strains isolated from different locations of the stomach in the same patient. H. pylori isolates were obtained from the antrum and body of the stomach. Genetic differences were examined by random amplified polymorphic DNA (RAPD) fingerprinting. Antibiotic resistance was assessed using the agar dilution method. Virulence factors were identified by polymerase chain reaction and DNA sequencing. Among 80 patients, co-infection by two H. pylori strains was detected in 10 patients. Among the 10 pairs of H. pylori strains, differences in antibiotic resistance patterns were detected in 7 pairs (clarithromycin, 1 patient; quinolone, 3 patients; metronidazole, 4 patients) and differences in virulence factors were detected in 5 pairs. The cagA virulence gene was detected in all 10 patients, and 2 patients had H. pylori strains with different EPIYA motifs. Differences in vacA genotypes were detected in 4 patients. Co-infection by two H. pylori strains was confi...Continue Reading

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Citations

May 16, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Wongwarut BoonyanugomolWoo-Kon Lee
Jun 14, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Parastoo SanieeFarideh Siavoshi

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Methods Mentioned

BETA
biopsies
biopsy
PCR
RAPD
electrophoresis
genotyping

Software Mentioned

CLC Main Workbench

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