The ancestral retinoic acid receptor was a low-affinity sensor triggering neuronal differentiation

Science Advances
Mette Handberg-ThorsagerMichael Schubert

Abstract

Retinoic acid (RA) is an important intercellular signaling molecule in vertebrate development, with a well-established role in the regulation of hox genes during hindbrain patterning and in neurogenesis. However, the evolutionary origin of the RA signaling pathway remains elusive. To elucidate the evolution of the RA signaling system, we characterized RA metabolism and signaling in the marine annelid Platynereis dumerilii, a powerful model for evolution, development, and neurobiology. Binding assays and crystal structure analyses show that the annelid retinoic acid receptor (RAR) binds RA and activates transcription just as vertebrate RARs, yet with a different ligand-binding pocket and lower binding affinity, suggesting a permissive rather than instructive role of RA signaling. RAR knockdown and RA treatment of swimming annelid larvae further reveal that the RA signal is locally received in the medial neuroectoderm, where it controls neurogenesis and axon outgrowth, whereas the spatial colinear hox gene expression in the neuroectoderm remains unaffected. These findings suggest that one early role of the new RAR in bilaterian evolution was to control the spatially restricted onset of motor and interneuron differentiation in the...Continue Reading

References

Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G AllenbyP Chambon
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·S G MansfieldW E Bollenbacher
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z KostrouchJ E Rall
Nov 26, 1998·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M MadenG E Jones
Jan 24, 2002·Nature·Pierre GermainHinrich Gronemeyer
May 1, 2002·Molecular Endocrinology·Pascal F EgeaDino Moras
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Nov 3, 2004·Nature Reviews. Drug Discovery·Hinrich GronemeyerVincent Laudet
Apr 26, 2005·Developmental Biology·Jennifer M DmetrichukRobert L Carlone
Aug 6, 2005·The Journal of Steroid Biochemistry and Molecular Biology·J E Balmer, R Blomhoff
May 12, 2006·Journal of Neurobiology·Rune Blomhoff, Heidi Kiil Blomhoff
Jun 21, 2006·Systematic Biology·Maria Anisimova, Olivier Gascuel
Jul 15, 2006·PLoS Genetics·Hector EscrivaVincent Laudet
Nov 30, 2006·Pharmacological Reviews·Pierre GermainHinrich Gronemeyer
Oct 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Virginie NahoumWilliam Bourguet
Jun 11, 2008·Nature Reviews. Genetics·Karen Niederreither, Pascal Dollé
Oct 22, 2008·Developmental Biology·Angela LinvilleThomas F Schilling
Nov 13, 2008·Genesis : the Journal of Genetics and Development·Florent Campo-PaysaaMichael Schubert
Mar 11, 2009·Journal of Visualized Experiments : JoVE·Jonathan N RosenJohn D Mably
Aug 5, 2009·Current Topics in Developmental Biology·Jeremy S Dasen, Thomas M Jessell
Jun 15, 2010·Nature Structural & Molecular Biology·Albane le MaireWilliam Bourguet
Jun 17, 2010·Methods in Molecular Biology·Maureen A Kane, Joseph L Napoli
Jan 5, 2011·Frontiers in Zoology·Antje Hl FischerDetlev Arendt
May 31, 2011·Biochimica Et Biophysica Acta·Joseph L Napoli
Aug 23, 2011·Genome Biology and Evolution·Ricard AlbalatMichael Schubert
Oct 11, 2011·Biochimica Et Biophysica Acta·Maureen A Kane
Oct 13, 2011·Molecular Systems Biology·Marco A Mendoza-ParraHinrich Gronemeyer
Jan 24, 2012·Methods in Enzymology·Erik MeijeringIhor Smal
Jun 5, 2012·The Journal of Biological Chemistry·Emmanuel MoutierIrwin Davidson
Dec 24, 2013·BMC Genomics·Markus ConzelmannGáspár Jékely
Jan 21, 2014·Current Biology : CB·Björn FuchsKonstantin Khalturin
Aug 15, 2014·Endocrinology·Juliana Gutierrez-MazariegosVincent Laudet

❮ Previous
Next ❯

Citations

May 3, 2019·The Journal of Biological Chemistry·Kenneth HuangGregory M K Poon
Jul 6, 2019·Development·Norbert B Ghyselinck, Gregg Duester
Apr 16, 2020·Biomolecules·Elza FonsecaL Filipe C Castro
May 1, 2019·Genesis : the Journal of Genetics and Development·Alysha JohnsonGaynor E Spencer
Sep 10, 2020·Biomolecules·Michael Schubert, Yann Gibert
Jan 15, 2021·Genes·Angelica MiglioliRémi Dumollard
Jan 22, 2020·Molecular and Cellular Endocrinology·Virginie Cuvillier-Hot, Alain Lenoir
Jul 6, 2021·Developmental Biology·Maryna P Lesoway, Jonathan Q Henry
Sep 29, 2021·EvoDevo·B Duygu ÖzpolatDetlev Arendt
Nov 4, 2021·Molecular Biology Reports·Sedigheh Momenzadeh, Mohammad-Saeid Jami
Dec 7, 2021·PLoS Computational Biology·Francesco MottesMichele Caselle

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoretic mobility shift
size exclusion chromatography
confocal reflection
PCR
Puller

Software Mentioned

phenix
MUSCLE
MacGDE
ContaMiner
Adobe Illustrator
TOPALi
SWISS
GraphPad Prism
Buccaneer
MoRDa

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.