PMID: 3582278Jan 1, 1987

The androgen receptor of the human endometrium

Endocrine Research
E K Muechler

Abstract

Available androgen binding to soluble proteins from the cytosol of human endometrium was studied using the dextran coated charcoal adsorption method and sucrose density centrifugation analysis. Specific binding of [3H]-5 alpha-dihydrotestosterone ([3H]-DHT) was observed with both methods. The apparent dissociation constant (Kd), for DHT binding is 1.3 +/- 0.2 (SEM) nM and the binding capacity 177 +/- 42 (SEM) fmol/mg protein. Sucrose density ultracentrifugation identifies specific [3H]-DHT binding that sediments at 4S and 8S. The stability of the androgen receptor in human endometrium is increased by the addition of 10% glycerol to the homogenization buffer. The addition of trypsin or pronase and heating at 60 degrees C reduces specific binding which demonstrates that the specific [3H]-DHT binder is a protein. The uptake of [3H] DHT in endometrial tissue minces indicated that 20% of the bound radioactivity was nuclear. Steroid specificity suggests that the binding protein from the uterus is specific for androgens. These observations indicate that androgen binding protein in the human uterus has the characteristics of the androgen receptor.

References

Feb 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·E K MuechlerG Mikhail
Nov 1, 1976·Journal of Steroid Biochemistry·A C NotidesE K Muechler
Nov 1, 1976·Journal of Steroid Biochemistry·W HeynsP de Moor
Jun 1, 1973·The Journal of Endocrinology·W I Mainwaring, E J Milroy
Dec 10, 1973·Biochemical and Biophysical Research Communications·R W Harrison, D O Toft
Oct 2, 1974·European Journal of Biochemistry·H Rochefort, F Lignon
Apr 1, 1973·Biology of Reproduction·P Corvol, C W Bardin
Aug 20, 1971·Biochemical and Biophysical Research Communications·G Giannopoulos
Dec 1, 1971·Hoppe-Seyler's Zeitschrift für physiologische Chemie·P W JungblutR K Wagner
Jan 1, 1983·The Journal of Clinical Endocrinology and Metabolism·D M WilbertJ D Wilson
Dec 1, 1982·Journal of Steroid Biochemistry·V HausknechtE Gurpide
Sep 1, 1983·Clinical Endocrinology·G JenkinG D Thorburn
Mar 1, 1982·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R Punnonen, A Lukola
Nov 1, 1980·The Journal of Clinical Endocrinology and Metabolism·E K Muechler, D Kohler
Aug 1, 1980·Endocrinologia Japonica·H WakimotoK Matsumoto

Related Concepts

Cytoplasmic Matrix
Endometrium
Androgen Receptor
Dihydroepitestosterone
Sterotate

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.