The angle between the anticodon and aminoacyl acceptor stems of yeast tRNA(Phe) is strongly modulated by magnesium ions

Biochemistry
M W Friederich, P J Hagerman

Abstract

Many tRNAs undergo tertiary folding transitions at temperatures well below the main thermally induced (hyperchromic) transition. Such transitions are essentially isochromic and isoenthalpic and display an absolute requirement for divalent cations; however, the nature of the structural transition is not known for any tRNA. Using a combination of transient electric birefringence (TEB) and gel electrophoretic measurements, we have characterized the influence of magnesium ions on the apparent angle between the anticodon and acceptor stems of a yeast tRNA(Phe) construct. TEB is a particularly sensitive method for quantifying the bends introduced in RNA by various nonhelix elements. In the current instance, the tRNA construct comprises an unmodified tRNA(Phe) molecule in which the anticodon and acceptor stems have been extended by approximately 70 bp to more effectively "report" the interstem angles. Upon the addition of sub-millimolar concentrations of magnesium ions, the tRNA core undergoes a substantial rearrangement in tertiary structure, passing from an open form with an apparent interstem angle of approximately 150 degrees to a conformation with an interstem angle of approximately 70 degrees (200 microM Mg2+). Further addition ...Continue Reading

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Citations

Mar 21, 1998·Current Opinion in Biotechnology·J Kjems, J Egebjerg
Dec 6, 2000·RNA·M R Hargittai, K Musier-Forsyth
Aug 23, 2008·Nucleic Acids Research·Ashley A Frazer-Abel, Paul J Hagerman
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M W FriederichP J Hagerman
Sep 29, 2004·Journal of Molecular Biology·Ashley A Frazer-Abel, Paul J Hagerman
Jun 30, 2000·Journal of Molecular Biology·D WittbergerR Schroeder
Jun 3, 2000·Journal of Molecular Biology·V K Misra, D E Draper
Jan 8, 1999·Journal of Molecular Biology·A A Frazer-Abel, P J Hagerman
Aug 15, 1998·Biological Chemistry·C M Spahn, K H Nierhaus
May 13, 2010·Biochemistry·Yuri Motorin, Mark Helm

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