The anodic hemoglobin of Anguilla anguilla. Molecular basis for allosteric effects in a root-effect hemoglobin.

The Journal of Biological Chemistry
A FagoRoy E Weber

Abstract

The functional and structural basis for the Root effect has been investigated in the anodic hemoglobin of the European eel, Anguilla anguilla. This hemoglobin exhibits a large Bohr effect, which is accounted for by oxygen-linked binding of seven to eight protons in the presence of GTP at pH 7.5. Oxygen equilibrium curves show nonlinear lower asymptote of Hill plots, indicating the occurrence of heme-heme interactions within the T state. Analysis of the curves according to the co-operon model (Brunori, M., Coletta, M., and Di Cera, E. (1986) Biophys. Chem. 23, 215-222) reveals that T state cooperativity is positive at high pH and in the stripped hemoglobin (where the T --> R allosteric transition is operative) and negative at low pH and in the presence of organic phosphate (where the molecule is locked in the low affinity structure), indicating site heterogeneity. The complete amino acid sequence of eel anodic hemoglobin has been established and compared with that of other fish hemoglobins. The presence of the Root effect correlates with a specific configuration of the alpha1beta2 switch interface, which at low pH would stabilize subunit ligation in the T state without changing the quaternary structure. We propose that the major...Continue Reading

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Citations

Nov 4, 2010·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Alessandra OlianasMariagiuseppina Pellegrini
May 2, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Barbara ManconiAlessandra Olianas
Mar 1, 2006·Fish Physiology and Biochemistry·Hung-Yueh YehPhillip H Klesius
Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·Bernd Pelster, Heinz Decker
Dec 22, 1999·Biochimica Et Biophysica Acta·T L TalaricoC J Stacey
Sep 6, 2012·Molecular Biology and Evolution·Juan C OpazoFederico G Hoffmann
Nov 24, 2004·Respiratory Physiology & Neurobiology·Roy E Weber, Angela Fago
Jun 22, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·P C de Souza, G O Bonilla-Rodriguez
Aug 20, 2003·The Journal of Biological Chemistry·Christian HundahlRoy E Weber
Jul 22, 2018·The Journal of Experimental Biology·Agnieszka JendroszekAngela Fago
Mar 27, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christian DamsgaardMark Bayley
Apr 5, 2000·The Journal of Biological Chemistry·R E WeberL Moens
Nov 24, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·B Pelster
May 30, 2003·The Journal of Biological Chemistry·Christina LarsenRoy E Weber
Jul 17, 2001·European Journal of Biochemistry·M TamburriniM Pellegrini
Sep 12, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jay F StorzRoy E Weber
May 24, 2006·The Journal of Biological Chemistry·Cinzia VerdeGuido di Prisco
Jan 26, 2013·The Journal of Experimental Biology·Christian DamsgaardRoy E Weber
Sep 21, 2007·Biochimica Et Biophysica Acta·Maria Cristina De RosaBruno Giardina

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