PMID: 9443379Aug 1, 1997Paper

The Anopheles gambiae tryptophan oxygenase gene expressed from a baculovirus promoter complements Drosophila melanogaster vermilion

Insect Biochemistry and Molecular Biology
N J BesanskyL Mcnitt

Abstract

An Anopheles gambiae cDNA encoding tryptophan oxygenase was placed under the control of the constitutive baculovirus promoter, ie-1. The chimeric construct, expressed transiently in vermilion (tryptophan oxygenase) mutants of Drosophila melanogaster, partially rescued adult eye color. The successful genetic complementation by this construct demonstrated both the proper function of the tryptophan oxygenase product and the effectiveness of the ie-1 promoter in directing expression of foreign genes in live insects. The functionality of An. gambiae tryptophan oxygenase in a higher fly fulfils predictions based on its structural conservation throughout millions of years of independent evolution.

References

Dec 1, 1995·Insect Biochemistry and Molecular Biology·M Q BenedictF H Collins
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·T MatsubaraJ C Burns
Nov 1, 1995·Insect Molecular Biology·N J BesanskyF H Collins
Jun 1, 1996·Insect Biochemistry and Molecular Biology·O MukabayireN J Besansky
Jul 1, 1996·Insect Biochemistry and Molecular Biology·L D WhiteD A O'Brochta
Sep 1, 1996·Insect Biochemistry and Molecular Biology·D A O'Brochta, P W Atkinson
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D A O'brochta, A M Handler

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Citations

Feb 13, 2001·Insect Biochemistry and Molecular Biology·A M Handler
Sep 17, 2002·Insect Molecular Biology·M D LorenzenR W Beeman

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