The anthelmintic efficacy of natural plant cysteine proteinases against the rat tapeworm Hymenolepis diminuta in vivo

Journal of Helminthology
F MansurJ M Behnke

Abstract

Hymenolepis diminuta is a natural parasite of the common brown rat Rattus norvegicus, and provides a convenient model system for the assessment of the anthelmintic activity of novel drugs against cestodes. The experiments described in this paper indicate that treatment of rats infected with H. diminuta with a supernatant extract of papaya latex, containing a mixture of four cysteine proteinases, was moderately efficacious, resulting in a significant, but relatively small, reduction in worm burden and biomass. However, faecal egg output was not affected by treatment. In our experiments these effects were only partially dose-dependent, although specific inhibition by E-64 confirmed the role of cysteine proteinases as the active principles in papaya latex affecting worm growth but not statistically reducing worm burden. Data collected for a further 7 days after treatment indicated that the effects of papaya latex supernatant on worm loss and on worm growth were not enhanced. Our findings provide a starting point for further refinement in formulation and delivery, or assessment of alternative natural plant-derived cysteine proteinases in efforts to develop these naturally occurring enzymes into broad-spectrum anthelmintics, with ef...Continue Reading

References

Feb 1, 1979·Experimental Parasitology·J M Behnke, H A Parish
Jul 1, 1976·Canadian Journal of Zoology·W S Evans, M Novak
Jul 25, 1992·The Veterinary Record·C J Proudman, G B Edwards
Nov 1, 1990·Biological Chemistry Hoppe-Seyler·D J ButtleA J Barrett
Apr 5, 1985·Biochimica Et Biophysica Acta·S ZuckerA J Barrett
Apr 1, 1970·Parasitology·C A Hopkins
Dec 1, 1994·Journal of Helminthology·F SatrijaS He
Apr 1, 1997·Molecular and Biochemical Parasitology·A C WhiteC L Chappell
Mar 11, 1998·Veterinary Parasitology·A MeanaM Gómez-Bautista
Apr 24, 1999·Advances in Parasitology·J TortJ P Dalton
Jun 11, 1999·Journal of Ethnopharmacology·A de AmorinM A Kaplan
Apr 26, 2000·The Journal of Parasitology·C P Read
Apr 26, 2000·The Journal of Parasitology·L S Roberts
Feb 19, 2002·Molecular and Biochemical Parasitology·M Sajid, J H McKerrow
Jun 1, 1959·Experimental Parasitology·J D SMYTH, J A CLEGG
Sep 3, 2008·Parasites & Vectors·Jerzy M BehnkeIan R Duce
Oct 13, 2012·International Journal for Parasitology·Jose-Luis Reyes HernandezDerek M McKay

❮ Previous
Next ❯

Citations

Jan 31, 2018·Frontiers in Microbiology·Anna SulimaDaniel Młocicki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.