Abstract
Disulfiram is commonly used for alcohol abuse; however, recent studies have revealed its potential as an anti-cancer treatment. This study investigated the effects of disulfiram on gastric cancer and its underlying mechanisms of action. The gastric cancer cell lines MKN-45 and SGC-7901 were used for all experiments. Cell proliferation was investigated using cell counting kit-8, cell migration and invasion were examined using Transwell assays, the proliferation and metastasis related proteins PCNA and MMP-2, respectively, were detected by ELISA. To explore the underlying molecular mechanisms, we also examined levels of proteins involved in the Wnt and NF-κB pathways by ELISA. Disulfiram significantly inhibited the proliferation, migration, and invasion of gastric cancer cells and decreased PCNA and MMP-2 levels. Additionally, disulfiram-treated MKN-45 and SGC-7901 cells showed reduced expression of Wnt, β-catenin, and NF-κB. Disulfiram regulates the Wnt and NF-κB pathways, and thus could be a potential treatment for managing gastric cancer.
References
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Feb 3, 2005·Cell Research·Roel Nusse
Aug 2, 2006·Molecular BioSystems·Zuben E SaunaSuresh V Ambudkar
Dec 2, 2006·Nature Reviews. Drug Discovery·Nick Barker, Hans Clevers
Feb 17, 2007·Cardiovascular Toxicology·Allan R Brasier
Apr 25, 2008·Nature Reviews. Cancer·Alexandra Klaus, Walter Birchmeier
Sep 3, 2010·The Prostate·Jianqing LinMichael A Carducci
Jan 21, 2011·Current Cancer Drug Targets·F R KonaA M Burger
May 23, 2012·Nature Medicine·Stephan P TenbaumHéctor G Palmer
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhao-Qiu WuStephen J Weiss
Mar 13, 2014·Cell Stem Cell·Antonija Kreso, John E Dick
Oct 27, 2014·Nature Cell Biology·Yihua WangXin Lu
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Oct 30, 2015·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Yoo Seob ShinEun Chang Choi
Sep 13, 2016·Oncogene·T ZhanM Boutros
Jan 31, 2017·Biochemical Pharmacology·Ming-Ju HsiehShun-Fa Yang
Feb 6, 2017·Molecular Cell·Katherine N Choe, George-Lucian Moldovan
May 19, 2017·British Journal of Pharmacology·Dario ZimmerliKonrad Basler
Sep 3, 2017·Cancer Letters·Juan CongXinhui Wang
Dec 7, 2017·Nature·Zdenek SkrottJiri Bartek
Jan 3, 2018·Acta Biomaterialia·Huacheng HePeisheng Xu
Mar 17, 2018·European Journal of Pharmacology·Xi WuChunfu Wu
Apr 18, 2018·Journal of Cellular Biochemistry·Na JinLiling Zhang
Citations
Jan 8, 2021·Frontiers in Oncology·Ling WangYumin Li
May 1, 2021·Pharmacology & Therapeutics·Devesh TewariAnupam Bishayee
Aug 28, 2021·Biomedicines·Supattra ChaithongyotMichael Naumann