The anti-apoptosis protein, survivin, mediates gastric epithelial cell cytoprotection against ethanol-induced injury via activation of the p34(cdc2) cyclin-dependent kinase

Journal of Cellular Physiology
Michael K JonesManith Norng

Abstract

The anti-apoptosis protein, survivin, promotes cell survival and mitosis. Recent studies have demonstrated that survivin is expressed in normal gastric mucosa. Using an in vitro model, we examined whether survivin plays a role in the cytoprotection produced in gastric mucosa by mild irritant ethanol (ETOH) against subsequent exposure to concentrated ETOH. Pre-treatment of rat gastric epithelial cells with 1% ETOH reduced cell death, in response to subsequent incubation with 5% ETOH, by 94% (P < 0.005). This pre-treatment also resulted in increased total and phosphorylated survivin protein levels by 180% (P < 0.0001) and 540% (P < 0.0002), respectively, which required the p34(cdc2) cell cycle-dependent kinase. The cytoprotective effect was abrogated upon siRNA knockdown of survivin protein levels. Further, overexpression of exogenous survivin resulted in significant cytoprotection by 62% (P < 0.02) in the absence of any pre-treatment. We further examined the in vivo relevance of these findings. In fasted rats, administration of 20% ETOH, which we found to be 93% (P < 0.0001) cytoprotective against 50% ETOH challenge, resulted in increased total and phosphorylated survivin protein levels by 234% (P < 0.001) and 214% (P < 0.02), r...Continue Reading

References

Jul 21, 1976·Biochimica Et Biophysica Acta·J G Culvenor, M J Weidemann
Apr 1, 1988·Gastroenterology·C J HawkeyB Filipowicz
Jul 1, 1983·The American Journal of Physiology·D L Stetson, P R Steinmetz
Jun 1, 1995·Alimentary Pharmacology & Therapeutics·J L Wallace, A W Tigley
Mar 1, 1993·Molecular and Cellular Biology·S Atherton-FesslerH Piwnica-Worms
Feb 10, 2000·The American Journal of Pathology·D S O'ConnorD C Altieri
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·D S O'ConnorD C Altieri
Oct 12, 2001·Journal of Physiology, Paris·B M Peskar
Feb 1, 2002·Human Immunology·Yoshihiro KobayashiYoshitaka Fujii
Aug 2, 2002·Cancer Cell·Daniel S O'ConnorDario C Altieri
Sep 11, 2002·Biochemical and Biophysical Research Communications·Masatoshi DeguchiTakeshi Nakano
Oct 24, 2002·Bioorganic & Medicinal Chemistry Letters·Vladimír KrystofMiroslav Strnad
May 15, 2003·Biochemical and Biophysical Research Communications·Shiun-Kwei ChiouAndrzej S Tarnawski
May 30, 2003·The EMBO Journal·Hiroyuki MarusawaJohn C Reed
Jul 29, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Dahn L ClemensDean J Tuma
Oct 8, 2003·Experimental Biology and Medicine·Shinji TsutsumiTohru Mizushima
Apr 24, 2004·Digestive Diseases and Sciences·Ken-Ichiro TanakaTohru Mizushima
Aug 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chih-Chuan ChangRu Chih C Huang
Dec 22, 2004·British Journal of Cancer·F Li
Jan 6, 2005·Gastroenterology·Shiun-Kwei ChiouAndrzej S Tarnawski
Jan 11, 2005·Current Medicinal Chemistry·K Gyires
May 6, 2005·Journal of Vascular Surgery·Hector F SimosaMichael S Conte
Dec 14, 2005·International Review of Cytology·Sally P Wheatley, Iain A McNeish
May 23, 2006·Trends in Cardiovascular Medicine·Michael S Conte, Dario C Altieri
Sep 12, 2006·Current Opinion in Cell Biology·Susanne M A LensRené H Medema
Apr 26, 2007·Cell Cycle·Sally P WheatleySusanne M A Lens

❮ Previous
Next ❯

Citations

Nov 14, 2008·Current Gastroenterology Reports·Amy Zhu, Jonathan Kaunitz
Apr 30, 2010·The Journal of Biological Chemistry·Rita Colnaghi, Sally P Wheatley
Apr 22, 2014·International Journal of Molecular Sciences·Xuejuan LiJie Ding
Dec 2, 2010·Alcoholism, Clinical and Experimental Research·M Katherine JungElizabeth J Kovacs
Aug 28, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yasuhiro TerasakiYuh Fukuda
Oct 8, 2014·Journal of Cellular Physiology·Annamaria CiminiMarcello Allegretti
Jan 6, 2009·Current Opinion in Gastroenterology·Maggie Ham, Jonathan D Kaunitz
Apr 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bishoy El-AaragHesham R El-Seedi
Jun 7, 2018·Journal of Neuro-oncology·B VoellgerR Firsching
May 9, 2013·Cancer Research·David J GrdinaRalph R Weichselbaum

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.